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dc.contributor.authorMajer, D.
dc.contributor.authorLewis, B.
dc.contributor.authorDevos, P.
dc.contributor.authorMithen, R.
dc.contributor.authorOliver, Richard
dc.date.accessioned2017-01-30T14:56:36Z
dc.date.available2017-01-30T14:56:36Z
dc.date.created2010-11-15T00:19:22Z
dc.date.issued1996
dc.identifier.citationMAJER D, LEWIS BG, DEVOS P, MITHEN RF & OLIVER RP (1996) Use of AFLPs to detect and measure variation in fungal populations. Mycological Research 100 1107-1111
dc.identifier.urihttp://hdl.handle.net/20.500.11937/41952
dc.identifier.doi10.1016/S0953-7562(96)80222-X
dc.description.abstract

A new PCR-based technique for the detection of inter- and intraspecific genetic variation has been tested on isolates of the fungal phytopathogens Cladosporium fulvum and Pyrenopeziza brassicae. The method is based on the selective PCR amplification of restriction fragments from digests of genomic DNA. We show that the technique is very efficient at detecting polymorphisms, even in species where very little variation could previously be found by RFLP analysis. 21 primer combinations were used on four isolates of P. brassicae, detecting a total of 162 polymorphisms (mean = 4·1 polymorphisms per primer combination per pair of isolates). Four primer combinations were used on eight isolates of C. fulvum, detecting a total of 32 polymorphisms (mean = 3·3 polymorphisms per primer combination per pair of isolates). Primer combinations varied in their ability to detect variation, ranging from 0 to 24 polymorphisms between P. brassicae isolates and 0 to 10 polymorphisms between C. fulvum isolates. AFLP fingerprints were highly reproducible and have great potential as a tool for evaluating genetic diversity of fungal pathogens

dc.titleUse of AFLPs to detect and measure variation in fungal populations
dc.typeJournal Article
curtin.note

A copy of this item may be available from Professor Richard Oliver

curtin.note

Email: Richard.oliver@curtin.edu.au

curtin.accessStatusFulltext not available
curtin.facultyDepartment of Environmental & Agriculture
curtin.facultySchool of Agriculture and Environment
curtin.facultyFaculty of Science and Engineering


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