Identification and quantification of native beta-casomorphins in Australian milk by LC-MS/MS and LC-HRMS
MetadataShow full item record
Beta-casomorphin 5 (BCM5) and beta-casomorphin 7 (BCM7) in 14 commercial pasteurised milks from around Australia were investigated using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and liquid chromatography-high resolution (Orbitrap) mass spectrometry (LC-HRMS). Collision induced dissociation HRMS was used to confirm the presence of BCM7 in milk extracts. The accurate mass-to-charge ratio ( m/ z) and relative abundance of BCM7 parent ion and fragments in milk extracts matched with those obtained from the analysis of a BCM7 standard solution. The deviation against the theoretical values of the measured m/ z of BCM7 (parent ion and fragments) all gave results below 5. ppm (relative error). BCM5 was below limit of detection (LOD) for all milks, while BCM7 was between 0.13 and 2.38 ng/g in 10 milks and below LOD in the others. Pasteurised milks containing the highest and lowest BCM7 levels were produced in Western Australia.
Showing items related by title, author, creator and subject.
Comparing different methods of human breast milk fortification using measured v. assumed macronutrient composition to target reference growth: a randomised controlled trialMcLeod, G.; Sherriff, Jill; Hartmann, P.; Nathan, E.; Geddes, D.; Simmer, K. (2015)The variable content of human breast milk suggests that its routine fortification may result in sub-optimal nutritional intakes and growth. In a pragmatic trial, we randomised infants born below 30 weeks of gestation to ...
Comparative effects of A1 versus A2 beta-casein on gastrointestinal measures: a blinded randomised cross-over pilot studyHo, S.; Woodford, K.; Kukuljan, S.; Pal, Sebely (2014)Background/objectives:At present, there is debate about the gastrointestinal effects of A1-type beta-casein protein in cows' milk compared with the progenitor A2 type. In vitro and animal studies suggest that digestion ...
Comparative Effects of A1 Versus A2 Beta-Casein on Gastrointestinal Measures: a Blinded Randomised Cross-Over Pilot StudyHo, Suleen; Woodford, K.; Kukuljan, S.; Pal, Sebely (2014)BACKGROUND/OBJECTIVES: At present, there is debate about the gastrointestinal effects of A1-type beta-casein protein in cows’ milk compared with the progenitor A2 type. In vitro and animal studies suggest that digestion ...