The inhibitory effect of Lu-Duo-Wei on carbon disulfide-induced generation of hydroxyl radicals
MetadataShow full item record
OBJECTIVE: To study the scavenging effects of Lu-Duo-Wei, thiourea, superoxide dismutase, and sodium azide on carbon disulfide-induced generation of hydroxyl radicals. METHODS: Phenanthroline-CuSO(4)-Vit C-H(2)O(2) chemiluminescence system (PHEN system) containing alcohol was established to probe the influence of various concentrations of carbon disulfide on hydroxyl radicals emission intensity and the scavenging effects of Lu-Duo-Wei and other antioxidants on carbon disulfide-induced hydroxyl radicals were observed. RESULTS: The average emission intensity of PHEN system containing alcohol appeared lower luminescence [91.03 x 10(3) (cp6s)] and longer time (75 s) to get the peak than the system without alcohol [96.11 x 10(3) (cp6s), 55 s]. The specific scavenger of hydroxyl radical, thiourea, showed clear inhibitory effect on the system. Carbon disulfide in the range of 40 - 160 mmol/L promoted the generation of hydroxyl radical, however, this effect could be efficiently inhibited by thiourea. 160 mmol/L carbon disulfide in PHEN system without copper seemed as an activator to promote the luminescence, while in PHEN system withdrawing phenanthroline appeared some weak action of luminescence agent at low concentration. Meanwhile, Lu-Duo-Wei may efficiently scavenge hydroxyl radicals induced by carbon disulfide in PHEN system but superoxide dismutase and sodium azide had little effects on the system. CONCLUSION: Carbon disulfide may induce PHEN system to generate hydroxyl radicals and Lu-Duo-Wei may efficiently scavenge these free radicals and play an important role in protection against oxidative injury induced by carbon disulfide.
Article in Chinese language
Showing items related by title, author, creator and subject.
The influence of Carbon Disulfide on Hydroxyl Free Radicals in Phenanthroline Chemiluminescence SystemJian, Le (2001)Objective: To clarify the effects of carbon disulfide (CS2) on hydroxyl free radical (*OH) generation in phenanthroline chemiluminescence system (Phen-system). Methods: The dynamic curve of various concentrations of CS2, ...
Jian, Le; Chiang, Huai (2001)We investigated the effects of carbon disulphide (CS2) on hydroxyl radical generation using the phenanthroline chemiluminescence system. The influence of CS2 on the phenanthroline chemiluminescence system without CuSO4 ...
Direct radical scavenging activity of benzbromarone provides beneficial antioxidant properties for hyperuricemia treatmentKadowaki, D.; Sakaguchi, S.; Miyamoto, Y.; Taguchi, K.; Muraya, N.; Narita, Y.; Sato, K.; Chuang, Victor; Maruyama, T.; Otagiri, M.; Hirata, S. (2015)Uric acid exerts an important antioxidant effect against external oxidative stress under physiological conditions. However, uric acid itself can increase oxidative stress via reduced nicotinamide adenine dinucleotide ...