A review on immobilised aptamers for high throughput biomolecular detection and screening
MetadataShow full item record
The discovery of Systematic Evolution of Ligands by Exponential Enrichment (SELEX) assay has led to the generation of aptamers from libraries of nucleic acids. Concomitantly, aptamer-target recognition and its potential biomedical applications have become a major research endeavour. Aptamers possess unique properties that make them superior biological receptors to antibodies with a plethora of target molecules. Some specific areas of opportunities explored for aptamer-target interactions include biochemical analysis, cell signalling and targeting, biomolecular purification processes, pathogen detection and, clinical diagnosis and therapy. Most of these potential applications rely on the effective immobilisation of aptamers on support systems to probe target species. Hence, recent research focus is geared towards immobilising aptamers as oligosorbents for biodetection and bioscreening. This article seeks to review advances in immobilised aptameric binding with associated successful milestones and respective limitations. A proposal for high throughput bioscreening using continuous polymeric adsorbents is also presented.
Showing items related by title, author, creator and subject.
Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separationAcquah, C.; Danquah, M.; Chan, Y.; Loo Chin Moy, Charles; Ongkudon, C.; Lau, John (2018)© 2018 Taylor & Francis The introduction of aptameric ligands onto disk-monolithic adsorbent, representing a unique strategy for convective isolation of target molecules with high specificity and selectivity, is ...
Tan, K.; Danquah, M.; Sidhu, Amandeep; Yon, L.; Ongkudon, C. (2016)© 2016 Bentham Science Publishers.The search for smart delivery systems for enhanced pre-clinical and clinical pharmaceutical delivery and cell targeting continues to be a major biomedical research endeavor owing to ...
Biophysical characterization of layer-by-layer synthesis of aptamer-drug microparticles for enhanced cell targetingTan, K.; Danquah, M.; Sidhu, Amandeep; Lau, Shiew Wei; Ongkudon, C. (2017)© 2017 American Institute of Chemical Engineers. Targeted delivery of drug molecules to specific cells in mammalian systems demonstrates a great potential to enhance the efficacy of current pharmaceutical therapies. ...