Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

Tong, S.; Lilliebridge, R.; Holt, D.; Coombs, Geoffrey; Currie, B.; Giffard, P.

doi:10.1016/j.diagmicrobio.2010.03.015

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Authors

Tong, S.

Lilliebridge, R.

Holt, D.

Coombs, Geoffrey

Currie, B.

Giffard, P.

Date

2010

Type

Journal Article

Metadata

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Citation

Tong, S. and Lilliebridge, R. and Holt, D. and Coombs, G. and Currie, B. and Giffard, P. 2010. Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis. Diagnostic Microbiology and Infectious Disease. 67 (4): pp. 399-401.

Source Title

Diagnostic Microbiology and Infectious Disease

DOI

10.1016/j.diagmicrobio.2010.03.015

ISSN

0732-8893

School

School of Biomedical Sciences

URI

http://hdl.handle.net/20.500.11937/43704

Collection

Curtin Research Publications

Abstract

We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. © 2010.