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    Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

    Access Status
    Fulltext not available
    Authors
    Tong, S.
    Lilliebridge, R.
    Holt, D.
    Coombs, Geoffrey
    Currie, B.
    Giffard, P.
    Date
    2010
    Type
    Journal Article
    
    Metadata
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    Citation
    Tong, S. and Lilliebridge, R. and Holt, D. and Coombs, G. and Currie, B. and Giffard, P. 2010. Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis. Diagnostic Microbiology and Infectious Disease. 67 (4): pp. 399-401.
    Source Title
    Diagnostic Microbiology and Infectious Disease
    DOI
    10.1016/j.diagmicrobio.2010.03.015
    ISSN
    0732-8893
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/43704
    Collection
    • Curtin Research Publications
    Abstract

    We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. © 2010.

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