Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis
Abstract
We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform. © 2010.
Citation
Tong, S. and Lilliebridge, R. and Holt, D. and Coombs, G. and Currie, B. and Giffard, P. 2010. Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis. Diagnostic Microbiology and Infectious Disease. 67 (4): pp. 399-401.
Source Title
Diagnostic Microbiology and Infectious Disease
ISSN
School
School of Biomedical Sciences
Collection
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