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    Production and characterization of human soluble CD83 fused with the fragment crystallizable region of human IgG1 in Pichia pastoris

    Access Status
    Fulltext not available
    Authors
    Yuan, Y.
    Wan, L.
    Chen, Younan
    Shi, M.
    Wang, C.
    Zhao, J.
    Lu, X.
    Wang, H.
    Lu, Y.
    Cheng, J.
    Date
    2013
    Type
    Journal Article
    
    Metadata
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    Citation
    Yuan, Y. and Wan, L. and Chen, Y. and Shi, M. and Wang, C. and Zhao, J. and Lu, X. et al. 2013. Production and characterization of human soluble CD83 fused with the fragment crystallizable region of human IgG1 in Pichia pastoris. Applied Microbiology and Biotechnology. 97 (21): pp. 9409-9417.
    Source Title
    Applied Microbiology and Biotechnology
    DOI
    10.1007/s00253-013-4732-1
    ISSN
    0175-7598
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/43816
    Collection
    • Curtin Research Publications
    Abstract

    The cell surface protein CD83 belongs to the immunoglobulin superfamily and is highly expressed on mature dendritic cells. The soluble form of CD83, sCD83, is a potential immune suppressor. In a previous study, recombinant soluble CD83 was expressed in Escherichia coli, resulting in a lack of functional glycosylation. Although eukaryotic cell systems for producing sCD83 offer the advantages of protein processing, folding, and posttranslational modification, these systems are complicated, expensive, and produce low levels of protein. To obtain more efficient expression of sCD83, we expressed human sCD83 fused with fragment crystallizable region of human IgG1 (hIgG1 Fc) in Pichia pastoris. Under the optimal conditions (time of induction, 48 h; inoculum density (OD 600), 80; concentration of methanol, 3.0 %; pH 7.0-8.0; concentration of casamino acid, 5.0 %), the purified human sCD83-hIgG1 Fc (hsCD83-Ig) fusion protein existed as dimers at 25-30 mg/L culture. Treatment with PNGase F showed that purified hsCD83-Ig was modified by N-linked glycosylation. Moreover, the hsCD83-Ig expressed in the P. pastoris system could suppress lymphocyte proliferation in ConA-stimulated and one-way mixed lymphocyte reaction systems. Thus, hsCD83-Ig expressed in P. pastoris is functional and may be used in experimental therapies for graft rejection, graft-versus-host disease, and autoimmune diseases. © 2013 Springer-Verlag Berlin Heidelberg.

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