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    Different digestion enzymes used for human pancreatic islet isolation: A mixed treatment comparison (MTC) meta-analysis

    Access Status
    Open access via publisher
    Authors
    Rheinheimer, J.
    Ziegelmann, P.
    Carlessi, Rodrigo
    Reck, L.
    Bauer, A.
    Leitão, C.
    Crispim, D.
    Date
    2015
    Type
    Journal Article
    
    Metadata
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    Citation
    Rheinheimer, J. and Ziegelmann, P. and Carlessi, R. and Reck, L. and Bauer, A. and Leitão, C. and Crispim, D. 2015. Different digestion enzymes used for human pancreatic islet isolation: A mixed treatment comparison (MTC) meta-analysis. Islets. 6 (4).
    Source Title
    Islets
    DOI
    10.4161/19382014.2014.977118
    ISSN
    1938-2014
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/4387
    Collection
    • Curtin Research Publications
    Abstract

    Collagenases are critical reagents determining yield and quality of isolated human pancreatic islets and may affect islet transplantation outcome. Some islet transplantation centers have compared 2 or more collagenase blends; however, the results regarding differences in quantity and quality of islets are conflicting. Thus, for the first time, a mixed treatment comparison (MTC) meta-analysis was carried out to compile data about the effect of different collagenases used for human pancreas digestion on islet yield, purity, viability and stimulation index (SI). Pubmed, Embase and Cochrane libraries were searched. Of 755 articles retrieved, a total of 15 articles fulfilled the eligibility criteria and were included in the MTC meta-analysis. Our results revealed that Vitacyte and Liberase MTF were associated with a small increase in islet yield (islet equivalent number/g pancreas) when compared with Sevac enzyme [standardized mean difference (95% credible interval - CrI) D -2.19 (-4.25 to -0.21) and -2.28 (-4.49 to -0.23), respectively]. However, all other enzyme comparisons did not show any significant difference regarding islet yield. Purity and viability percentages were not significantly different among any of the analyzed digestion enzymes. Interestingly, Vitacyte and Serva NB1 were associated with increased SI when compared with Liberase MTF enzyme [unstandardized weighted mean difference (95% CrI) D -1.69 (-2.87 to -0.51) and -1.07 (-1.79 to -0.39), respectively]. In conclusion, our MTC meta-analysis suggests that the digestion enzymes currently being used for islet isolation works with similar efficiency regarding islet yield, purity and viability; however, Vitacyte and Serva NB1 enzymes seem to be associated with an improved SI as compared with Liberase MTF.

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