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    Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome

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    Fulltext not available
    Authors
    Tan, H.
    Mooij, M.
    Barret, M.
    Hegarty, P.
    Harrington, C.
    Dobson, A.
    O'Gara, Fergal
    Date
    2014
    Type
    Journal Article
    
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    Citation
    Tan, H. and Mooij, M. and Barret, M. and Hegarty, P. and Harrington, C. and Dobson, A. and O'Gara, F. 2014. Identification of Novel Phytase Genes from an Agricultural Soil-Derived Metagenome. Journal of Microbiology and Biotechnology. 24 (1): pp. 113-118.
    Source Title
    Journal of Microbiology and Biotechnology
    DOI
    10.4014/jmb.1307.07007
    ISSN
    1017-7825
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/4398
    Collection
    • Curtin Research Publications
    Abstract

    Environmental microorganisms are emerging as an important source of new enzymes for wide-scale industrial application. In this study, novel phytase genes were identified from a soil microbial community. For this, a function-based screening approach was utilized for the identification of phytase activity in a metagenomic library derived from an agricultural soil. Two novel phytases were identified. Interestingly, one of these phytases is an unusual histidine acid phosphatase family phytase, as the conserved motif of the active site of PhyX possesses an additional amino acid residue. The second phytase belongs to a new type, which is encoded by multiple open reading frames (ORFs) and is different to all phytases known to date, which are encoded by a single ORF.

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