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    Stripping voltammetric detection of insulin at liquid–liquid microinterfaces in the presence of bovine albumin

    192855_95486_75409.pdf (589.9Kb)
    Access Status
    Open access
    Authors
    O'Sullivan, S.
    De Eulate, E.
    Yuen, Y.
    Helmerhorst, Erik
    Arrigan, Damien
    Date
    2013
    Type
    Journal Article
    
    Metadata
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    Citation
    O'Sullivan, Shane and De Eulate, Eva and Yuen, Yiu Hang and Helmerhorst, Erik and Arrigan, Damien. 2013. Stripping voltammetric detection of insulin at liquid–liquid microinterfaces in the presence of bovine albumin. Analyst. 138: pp. 6192-6196.
    Source Title
    Analyst
    DOI
    10.1039/C3AN01123B
    ISSN
    0003-2654
    URI
    http://hdl.handle.net/20.500.11937/43997
    Collection
    • Curtin Research Publications
    Abstract

    Electrochemistry at the interface between two immiscible electrolyte solutions (ITIES) provides a platform for label-free detection of biomolecules. In this study, adsorptive stripping voltammetry (AdSV) was implemented at an array of microscale ITIES for the detection of the antidiabetic hormone insulin. By exploiting the potential-controlled adsorption of insulin at the ITIES, insulin was detected at 10 nM via subsequent voltammetric desorption. This is the lowest detected concentration reported to-date for a protein by electrochemistry at the ITIES. Surface coverage calculations indicate that between 0.1 and 1 monolayer of insulin forms at the interface over the 10 – 1000 nM concentration range of the hormone. In a step toward assessment of selectivity, the optimum adsorption potentials for insulin and albumin were determined to be 0.900 V and 0.975 V, respectively. When present in an aqueous mixture with albumin, insulin was detected by tuning the adsorption potential to 0.9 V, albeit with reduced sensitivity. This provides the first example of selective detection of one protein in the presence of another by exploiting optimal adsorption potentials. The results presented here provide a route to the improvement of detection limits and achievement of selectivity for protein detection by electrochemistry at the ITIES.

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