Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Analysis of free amino acids in natural waters by liquid chromatography-tandem mass spectrometry

    204859_204859.pdf (812.4Kb)
    Access Status
    Open access
    Authors
    How, Zuo tong
    Busetti, Francesco
    Linge, Kathryn
    Kristiana, Ina
    Joll, Cynthia
    Charrois, Jeffrey
    Date
    2014
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    How, Z.T. and Busetti, F. and Linge, K. and Kristiana, I. and Joll, C. and Charrois, J. 2014. Analysis of free amino acids in natural waters by liquid chromatography-tandem mass spectrometry. Journal of Chromatography A. 1370: pp. 135-146.
    Source Title
    Journal of Chromatography A
    DOI
    10.1016/j.chroma.2014.10.040
    ISSN
    0021-9673
    School
    Curtin Water Quality Research Centre (Industry Research Centre)
    Remarks

    NOTICE: this is the author’s version of a work that was accepted for publication in the Journal of Chromatography A. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in the Journal of Chromatography A, Vol.1370 (2014). DOI: http://doi.org/10.1016/j.chroma.2014.10.040

    URI
    http://hdl.handle.net/20.500.11937/44212
    Collection
    • Curtin Research Publications
    Abstract

    This paper reports a new analytical method for the analysis of 18 amino acids in natural waters using solid-phase extraction (SPE) followed by liquid chromatography-electrospray tandem mass spectrometry (LC–MS/MS) operated in multiple reaction monitoring mode. Two different preconcentration methods, solid-phase extraction and concentration under reduced pressure, were tested in development of this method. Although concentration under reduced pressure provided better recoveries and method limits of detection for amino acids in ultrapure water, SPE was a more suitable extraction method for real samples due to the lower matrix effects for this method. Even though the strong cation exchange resin used in SPE method introduced exogenous matrix interferences into the sample extracts (inorganic salt originating from the acid–base reaction during the elution step), the SPE method still incorporates a broad sample clean-up and minimised endogenous matrix effects by reducing interferences originating from real water samples. The method limits of quantification (MLQ) for the SPE LC–MS/MS method in ultrapure water ranged from 0.1 to 100 μg L−1 as N for the different amino acids. The MLQs of the early eluting amino acids were limited by the presence of matrix interfering species, such as inorganic salts in natural water samples. The SPE LC–MS/MS method was successfully applied to the analysis of amino acids in 3 different drinking water source waters: the average total free amino acid content in these waters was found to be 19 μg L−1 as N, while among the 18 amino acids analysed, the most abundant amino acids were found to be tyrosine, leucine and isoleucine.

    Related items

    Showing items related by title, author, creator and subject.

    • Urolithiasis: occurrence and function of intracrystalline proteins in calcium oxalate monohydrate crystals
      Fleming, David Elliot (2004)
      The broad aim of the work presented in this thesis was to examine the relationship between the mineral and organic phases of calcium oxalate monohydrate (COM) crystals, which are the principal components of human kidney ...
    • Characterisation of aquatic natural organic matter by micro-scale sealed vessel pyrolysis
      Berwick, Lyndon (2009)
      The analytical capacity of MSSV pyrolysis has been used to extend the structural characterisation of aquatic natural organic matter (NOM). NOM can contribute to various potable water issues and is present in high ...
    • Chemical markers of decomposition for forensic science
      Swann, Lisa (2011)
      This thesis describes investigations that were carried out to determine the chemical compounds produced during the decomposition of an animal model in the absence of a soil matrix. In order to do this, several analytical ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.