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    A quantitative PCR approach to determine gene copy number

    Access Status
    Fulltext not available
    Authors
    Solomon, P.
    Ipcho, S.
    Hane, J.
    Tan, Kar-Chun
    Oliver, Richard
    Date
    2008
    Type
    Journal Article
    
    Metadata
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    Citation
    SOLOMON PS, IPCHO SVS, HANE JK, TAN K-C, OLIVER RP. (2008) A quantitative PCR approach to determine gene copy number. Fungal Genetics Reports 55 5-8
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/44274
    Collection
    • Curtin Research Publications
    Abstract

    Here, we report on the use of quantitative PCR (qPCR) to determine gene copy number in filamentous fungi. Using the sequenced dothideomycete Stagonospora nodorum, qPCR was used to unequivocally confirm the presence of single, two and three copy regions as predicted by in silico PCR. Further validation of the technique was demonstrated by verifying the copy numbers of introduced gene cassettes in previously characterised transformants of S. nodorum. Apart from increased sensitivity, this technique offers a high-throughput alternative to Southern blots for determining gene copy number, a significant factor when screening fungal mutants and transformants.

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