Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Evaluation of a Multilocus Indel DNA Region for the Detection of the Wheat Tan Spot Pathogen Pyrenophora tritici-repentis

    Access Status
    Fulltext not available
    Authors
    See, Pao Theen
    Moffat, Caroline
    Morina, J.
    Oliver, Richard
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    See, P.T. and Moffat, C. and Morina, J. and Oliver, R. 2016. Evaluation of a Multilocus Indel DNA Region for the Detection of the Wheat Tan Spot Pathogen Pyrenophora tritici-repentis. Plant Disease. 100 (11): pp. 2215-2225.
    Source Title
    Plant Disease
    DOI
    10.1094/PDIS-03-16-0262-RE
    Additional URLs
    http://apsjournals.apsnet.org/doi/10.1094/PDIS-03-16-0262-RE
    ISSN
    0191-2917
    School
    Centre for Crop Disease Management
    URI
    http://hdl.handle.net/20.500.11937/4504
    Collection
    • Curtin Research Publications
    Abstract

    Tan spot or yellow (leaf) spot disease of wheat (Triticum spp.) is caused by Pyrenophora tritici-repentis, a necrotrophic fungal pathogen that is wide-spread throughout the main wheat-growing regions in the world. This disease is currently the single most economically important crop disease in Australia. IN this study, a real-time quantitative polymerase chain reaction (qPCR) assay was developed as a diagnostic tool to detect the pathogen on wheat foliar tissue. A multicopy locus (PtrMulti) present in the P. tritici-repentis genome was assessed for its suitability as a qPCR probe. The primer pair PtrMulti_F/R that targets the region was evaluated with respect to species specificity and sensitivity. A PtrMulti SYBR qPCR assay was developed and proved to be suitable for the identification and relative quantification of P. tritici-repentis with a detection limi of DNA levels at <0.1 pg. Variation of the PtrMulti copy number between the geographical representatives of P. tritici-repentis strains examined was minimal, with the range of 63 to 85 copies per genome. For naturally infected wheat field samples, the incidence of P. tritici-repentis DNA on leaves quantified by qPCR varied up to 1,000-fold difference in the concentration, with a higher incidence of DNA on the younger leaves in the absence of visible tan spot lesions. These results demonstrate the potential of PtrMulti probe to be used for early detection and rapid screening of tan spot disease on wheat plants.

    Related items

    Showing items related by title, author, creator and subject.

    • Emergence of tan spot disease caused by toxigenic Pyrenophora tritici-repentis in Australia is not associated with increased deployment of toxin-sensitive cultivars
      Oliver, Richard; Lord, M.; Rybak, K.; Faris, J.; Solomon, P. (2008)
      The wheat disease tan (or yellow leaf) spot, caused by Pyrenophora tritici-repentis, was first described in the period 1934 to 1941 in Canada, India, and the United States. It was first noted in Australia in 1953 and only ...
    • The emergence of tan spot disease caused by toxigenic Pyrenophora tritici-repentis in Australia is not associated with increased deployment of toxin-sensitive cultivars
      Oliver, Richard; Lord, M.; Rybak, K.; Faris, J.; Solomon, P. (2008)
      The wheat disease tan (or yellow leaf) spot, caused by Pyrenophora tritici-repentis, was first described in the period 1934 to 1941 in Canada, India, and the United States. It was first noted in Australia in 1953 and only ...
    • Hidden in plain sight: a molecular field survey of three wheat leaf blotch fungal diseases in North-Western Europe shows co-infection is widespread.
      Justesen, Annemarie; Corsi, Beatrice; Ficke, Andrea; Hartl, Lorenz; Holdgate, Sarah; Jørgensen, Lise; Lillemo, Morten; Lin, Min; Mackay, Ian; Mohler, Volker; Stadlmeier, Melanie; Tan, Kar-Chun ; Turner, Judith; Oliver, Richard; Cockram, James (2021)
      Wheat (Triticum aestivum L.) yields are commonly affected by foliar infection by fungal pathogens. Of these, three wheat leaf blotch fungal diseases, septoria nodorum blotch (SNB), tan spot (TS) and septoria tritici blotch ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.