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dc.contributor.authorChua, K.
dc.contributor.authorSeemann, T.
dc.contributor.authorHarrison, P.
dc.contributor.authorMonagle, S.
dc.contributor.authorKorman, T.
dc.contributor.authorJohnson, P.
dc.contributor.authorCoombs, Geoffrey
dc.contributor.authorHowden, B.
dc.contributor.authorDavies, J.
dc.contributor.authorHowden, B.
dc.contributor.authorStinear, T.
dc.date.accessioned2017-01-30T15:18:33Z
dc.date.available2017-01-30T15:18:33Z
dc.date.created2014-11-19T01:13:21Z
dc.date.issued2011
dc.identifier.citationChua, K. and Seemann, T. and Harrison, P. and Monagle, S. and Korman, T. and Johnson, P. and Coombs, G. et al. 2011. The Dominant Australian Community-Acquired Methicillin-Resistant Staphylococcus aureus Clone ST93-IV [2B] Is Highly Virulent and Genetically Distinct. PLoS ONE. 6 (10): e25887.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/45106
dc.identifier.doi10.1371/journal.pone.0025887
dc.description.abstract

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) USA300 has spread rapidly across North America, and CA-MRSA is also increasing in Australia. However, the dominant Australian CA-MRSA strain, ST93-IV [2B] appears distantly related to USA300 despite strikingly similar clinical and epidemiological profiles. Here, we compared the virulence of a recent Australian ST93 isolate (JKD6159) to other MRSA, including USA300, and found that JKD6159 was the most virulent in a mouse skin infection model. We fully sequenced the genome of JKD6159 and confirmed that JKD6159 is a distinct clone with 7616 single nucleotide polymorphisms (SNPs) distinguishing this strain from all other S. aureus genomes. Despite its high virulence there were surprisingly few virulence determinants. However, genes encoding a-hemolysin, Panton-Valentine leukocidin (PVL) and a-type phenol soluble modulins were present. Genome comparisons revealed 32 additional CDS in JKD6159 but none appeared to encode new virulence factors, suggesting that this clone’s enhanced pathogenicity could lie within subtler genome changes, such as SNPs within regulatory genes. To investigate the role of accessory genome elements in CA-MRSA epidemiology, we next sequenced three additional Australian non-ST93 CA-MRSA strains and compared them with JKD6159, 19 completed S. aureus genomes and 59 additional S. aureus genomes for which unassembled genome sequence data was publicly available (82 genomes in total). These comparisons showed that despite its distinctive genotype, JKD6159 and other CA-MRSA clones (including USA300) share a conserved repertoire of three notable accessory elements (SSCmecIV, PVL prophage, and pMW2). This study demonstrates that the genetically distinct ST93 CA-MRSA from Australia is highly virulent. Our comparisons of geographically and genetically diverse CA-MRSA genomes suggest that apparent convergent evolution in CA-MRSA may be better explained by the rapid dissemination of a highly conserved accessory genome from a common source.

dc.publisherPublic Library of Science
dc.titleThe Dominant Australian Community-Acquired Methicillin-Resistant Staphylococcus aureus Clone ST93-IV [2B] Is Highly Virulent and Genetically Distinct
dc.typeJournal Article
dcterms.source.volume6
dcterms.source.number10
dcterms.source.startPagee25887
dcterms.source.endPage1
dcterms.source.issn19326203
dcterms.source.titlePLoS ONE
curtin.note

This article is published under the Open Access publishing model and distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ Please refer to the licence to obtain terms for any further reuse or distribution of this work.

curtin.accessStatusOpen access


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