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    A Transcriptomic Signature of Mouse Liver Progenitor Cells

    246353_246353.pdf (7.704Mb)
    Access Status
    Open access
    Authors
    Passman, A.
    Low, J.
    London, R.
    Tirnitz-Parker, Nina
    Miyajima, A.
    Tanaka, M.
    Strick-Marchand, H.
    Darlington, G.
    Finch-Edmondson, M.
    Ochsner, S.
    Zhu, C.
    Whelan, J.
    Callus, B.
    Yeoh, G.
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Passman, A. and Low, J. and London, R. and Tirnitz-Parker, N. and Miyajima, A. and Tanaka, M. and Strick-Marchand, H. et al. 2016. A Transcriptomic Signature of Mouse Liver Progenitor Cells. Stem Cells International. 2016: Article ID 5702873.
    Source Title
    Stem Cells International
    DOI
    10.1155/2016/5702873
    School
    School of Biomedical Sciences
    Remarks

    This open access article is distributed under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    URI
    http://hdl.handle.net/20.500.11937/45175
    Collection
    • Curtin Research Publications
    Abstract

    Liver progenitor cells (LPCs) can proliferate extensively, are able to differentiate into hepatocytes and cholangiocytes, and contribute to liver regeneration. The presence of LPCs, however, often accompanies liver disease and hepatocellular carcinoma (HCC), indicating that they may be a cancer stem cell. Understanding LPC biology and establishing a sensitive, rapid, and reliable method to detect their presence in the liver will assist diagnosis and facilitate monitoring of treatment outcomes in patients with liver pathologies. A transcriptomic meta-analysis of over 400 microarrays was undertaken to compare LPC lines against datasets of muscle and embryonic stem cell lines, embryonic and developed liver (DL), and HCC. Three gene clusters distinguishing LPCs from other liver cell types were identified. Pathways overrepresented in these clusters denote the proliferative nature of LPCs and their association with HCC. Our analysis also revealed 26 novel markers, LPC markers, including Mcm2 and Ltbp3, and eight known LPC markers, including M2pk and Ncam. These markers specified the presence of LPCs in pathological liver tissue by qPCR and correlated with LPC abundance determined using immunohistochemistry. These results showcase the value of global transcript profiling to identify pathways and markers that may be used to detect LPCs in injured or diseased liver.

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