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dc.contributor.authorO'Brien, Frances
dc.contributor.authorEto, K.
dc.contributor.authorMurphy, R.
dc.contributor.authorFairhurst, H.
dc.contributor.authorCoombs, Geoffrey
dc.contributor.authorGrubb, W.
dc.contributor.authorRamsay, Joshua
dc.date.accessioned2017-01-30T15:22:09Z
dc.date.available2017-01-30T15:22:09Z
dc.date.created2015-10-29T04:09:34Z
dc.date.issued2015
dc.identifier.citationO'Brien, F. and Eto, K. and Murphy, R. and Fairhurst, H. and Coombs, G. and Grubb, W. and Ramsay, J. 2015. Origin-of-transfer sequences facilitate mobilisation of non-conjugative antimicrobial-resistance plasmids in Staphylococcus aureus. Nucleic Acids Research. 43 (16): pp. 7971-7983.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/45635
dc.identifier.doi10.1093/nar/gkv755
dc.description.abstract

Staphylococcus aureus is a common cause of hospital, community and livestock-associated infections and is increasingly resistant to multiple antimicrobials. A significant proportion of antimicrobial-resistance genes are plasmid-borne, but only a minority of S. aureus plasmids encode proteins required for conjugative transfer or Mob relaxase proteins required for mobilisation. The pWBG749 family of S. aureus conjugative plasmids can facilitate the horizontal transfer of diverse antimicrobial-resistance plasmids that lack Mob genes. Here we reveal that these mobilisable plasmids carry copies of the pWBG749 origin-of-transfer (oriT) sequence and that these oriT sequences facilitate mobilisation by pWBG749. Sequences resembling the pWBG749 oriT were identified on half of all sequenced S. aureus plasmids, including the most prevalent large antimicrobial-resistance/virulence-gene plasmids, pIB485, pMW2 and pUSA300HOUMR. oriT sequences formed five subfamilies with distinct inverted-repeat-2 (IR2) sequences. pWBG749-family plasmids encoding each IR2 were identified and pWBG749 mobilisation was found to be specific for plasmids carrying matching IR2 sequences. Specificity of mobilisation was conferred by a putative ribbon-helix-helix-protein gene smpO. Several plasmids carried 2–3 oriT variants and pWBG749-mediated recombination occurred between distinct oriT sites during mobilisation. These observations suggest this relaxase-in trans mechanism of mobilisation by pWBG749-family plasmids is a common mechanism of plasmid dissemination in S. aureus.

dc.publisherOxford University Press
dc.titleOrigin-of-transfer sequences facilitate mobilisation of non-conjugative antimicrobial-resistance plasmids in Staphylococcus aureus
dc.typeJournal Article
dcterms.source.volume43
dcterms.source.number16
dcterms.source.startPage7971
dcterms.source.endPage7983
dcterms.source.issn0305-1048
dcterms.source.titleNucleic Acids Research
curtin.note

This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusOpen access


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