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dc.contributor.authorMunyard, Kylie
dc.contributor.authorLedger, J.
dc.contributor.authorLee, C.
dc.contributor.authorBabra, C.
dc.contributor.authorGroth, David
dc.contributor.editorAlex Safari, Bill Pattie, Barrie Restall
dc.date.accessioned2017-01-30T15:26:48Z
dc.date.available2017-01-30T15:26:48Z
dc.date.created2009-11-29T20:01:39Z
dc.date.issued2009
dc.identifier.citationMunyard, K.A. and Ledger, J. M. and Lee, C. Y. and Babra, C. and Groth, D. M. 2009. Association for the Advancement of Animal Breeding and Genetics 18th Biennial Conference, Sep 28 2009: Isolation and Characterization of Alpaca Tetranucleotide Microsatellite Markers. Barossa Valley, South Australia: Association for the Advancement of Animal Breeding and Genetics.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/46352
dc.description.abstract

Hybridisation-capture was used to create 12 unique alpaca DNA libraries each enriched for a different tetranucleotide microsatellite motif. Two hundred and forty nine microsatellites were found, of which 26 were polymorphic (motifs GGAT, GTTT and GCAC). Nine markers were fully characterised on 45 samples. Allele numbers ranged from 6 (Locus P135) to 12 (loci P149 and PCTD17). There was no evidence of linkage disequilibrium (p = 0.064 - 1) or deviation from Hardy-Weinberg equilibrium (p = 1). Polymorphic information content ranged from 0.48 to 0.82. When combined, the markers had an exclusion probability of 97.7%. These markers will be useful for parentage determination (especially if combined into a multiplex) and will add to the pool of markers available for mapping of desirous or deleterious traits in alpacas.

dc.publisherAssociation for the Advancement of Animal Breeding and Genetics
dc.relation.urihttp://www.aaabg.org/proceedings18/index.html
dc.subjectmicrosatellite
dc.subjectalpaca
dc.titleIsolation and Characterization of Alpaca Tetranucleotide Microsatellite Markers
dc.typeConference Paper
dcterms.source.startPage620
dcterms.source.endPage623
dcterms.source.titleProceedings of the Eighteenth conference: Matching Genetics and Environment: a New look at an old topic
dcterms.source.seriesProceedings of the Eighteenth conference: Matching Genetics and Environment: a New look at an old topic
dcterms.source.isbn978-0-646-52103-9
dcterms.source.conferenceAssociation for the Advancement of Animal Breeding and Genetics 18th Biennial Conference
dcterms.source.conference-start-dateSep 28 2009
dcterms.source.conferencelocationBarossa Valley, South Australia
dcterms.source.placeSouth Australia
curtin.accessStatusOpen access
curtin.facultyFaculty of Health Sciences
curtin.facultySchool of Biomedical Sciences


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