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    The RNA chaperone, Hfq, controls two luxR-type regulators and plays a key role in pathogenesis and production of antibiotics in Serratia sp. ATCC 39006

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    Authors
    Wilf, N.
    Williamson, N.
    Ramsay, Joshua
    Poulter, S.
    Bandyra, K.
    Salmond, G.
    Date
    2011
    Type
    Journal Article
    
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    Citation
    Wilf, N. and Williamson, N. and Ramsay, J. and Poulter, S. and Bandyra, K. and Salmond, G. 2011. The RNA chaperone, Hfq, controls two luxR-type regulators and plays a key role in pathogenesis and production of antibiotics in Serratia sp. ATCC 39006. Environmental Microbiology. 13 (10): pp. 2649-2666.
    Source Title
    Environmental Microbiology
    DOI
    10.1111/j.1462-2920.2011.02532.x
    ISSN
    1462-2912
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/46905
    Collection
    • Curtin Research Publications
    Abstract

    Serratia sp. ATCC 39006 (S39006) is a Gram-negative bacterium that is virulent in plant (potato) and animal (Caenorhabditis elegans) models. It produces two secondary metabolite antibiotics, a prodigiosin and a carbapenem, and the exoenzymes, pectate lyase and cellulase. A complex regulatory network that includes quorum sensing (QS) controls production of prodigiosin. While many aspects of the regulation of the metabolites and exoenzymes are well understood, the potential role in this network of the RNA chaperone Hfq and dependent small regulatory RNAs has not been characterized. Hfq is an RNA chaperone involved in post-transcriptional regulation that plays a key role in stress response and virulence in diverse bacterial species. To explore whether Hfq-dependent processes might contribute to the regulation of antibiotic production we constructed an S39006 ?hfq mutant. Production of prodigiosin and carbapenem was abolished in this mutant strain, while production of the QS signalling molecule, butanoyl homoserine lactone (BHL), was unaffected. Using transcriptional fusions, we found that Hfq regulates the QS response regulators, SmaR and CarR. Additionally, exoenzyme production and swimming motility were decreased in a ?hfq mutant, and virulence was attenuated in potato and C. elegans models. These results suggest that an Hfq-dependent pathway is involved in the regulation of virulence and secondary metabolite production in S39006. © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.

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