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dc.contributor.authorFortes, M.
dc.contributor.authorMarzuca-Nassr, G.
dc.contributor.authorVitzel, K.
dc.contributor.authorDa Justa Pinheiro, C.
dc.contributor.authorNewsholme, Philip
dc.contributor.authorCuri, R.
dc.date.accessioned2017-01-30T15:36:17Z
dc.date.available2017-01-30T15:36:17Z
dc.date.created2016-05-22T19:30:26Z
dc.date.issued2016
dc.identifier.citationFortes, M. and Marzuca-Nassr, G. and Vitzel, K. and Da Justa Pinheiro, C. and Newsholme, P. and Curi, R. 2016. Housekeeping proteins: How useful are they in skeletal muscle diabetes studies and muscle hypertrophy models?. Analytical Biochemistry. 504: pp. 38-40.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/47892
dc.identifier.doi10.1016/j.ab.2016.03.023
dc.description.abstract

The use of Western blot analysis is of great importance in research, and the measurement of housekeeping proteins is commonly used for loading controls. However, Ponceau S staining has been shown to be an alternative to analysis of housekeeping protein levels as loading controls in some conditions. In the current study, housekeeping protein levels were measured in skeletal muscle hypertrophy and streptozotocin-induced diabetes experimental models. The following housekeeping proteins were investigated: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-actin, α-tubulin, γ-tubulin, and α-actinin. Evidence is presented that Ponceau S is more reliable than housekeeping protein levels for specific protein quantifications in Western blot analysis.

dc.publisherAcademic Press
dc.titleHousekeeping proteins: How useful are they in skeletal muscle diabetes studies and muscle hypertrophy models?
dc.typeJournal Article
dcterms.source.volume504
dcterms.source.startPage38
dcterms.source.endPage40
dcterms.source.issn0003-2697
dcterms.source.titleAnalytical Biochemistry
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusFulltext not available


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