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dc.contributor.authorDeplazes, Evelyne
dc.contributor.authorLouhivuori, M.
dc.contributor.authorJayatilaka, D.
dc.contributor.authorMarrink, S.
dc.contributor.authorCorry, B.
dc.identifier.citationDeplazes, E. and Louhivuori, M. and Jayatilaka, D. and Marrink, S. and Corry, B. 2012. Structural Investigation of MscL Gating Using Experimental Data and Coarse Grained MD Simulations. PLoS Computational Biology. 8 (9): Article ID e1002683.

The mechanosensitive channel of large conductance (MscL) has become a model system in which to understand mechanosensation, a process involved in osmoregulation and many other physiological functions. While a high resolution closed state structure is available, details of the open structure and the gating mechanism remain unknown. In this study we combine coarse grained simulations with restraints from EPR and FRET experiments to study the structural changes involved in gating with much greater level of conformational sampling than has previously been possible. We generated a set of plausible open pore structures that agree well with existing open pore structures and gating models. Most interestingly, we found that membrane thinning induces a kink in the upper part of TM1 that causes an outward motion of the periplasmic loop away from the pore centre. This previously unobserved structural change might present a new mechanism of tension sensing and might be related to a functional role in osmoregulation.

dc.titleStructural Investigation of MscL Gating Using Experimental Data and Coarse Grained MD Simulations
dc.typeJournal Article
dcterms.source.titlePLoS Computational Biology

This open access article is distributed under the Creative Commons license

curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusOpen access

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