Review of fluorescent standards for calibration of in situ fluorometers: Recommendationsapplied in coastal and ocean observing programs
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Fluorometers are widely used in ecosystem observing to monitor fluorescence signals from organic compounds, as well as to infer geophysical parameters such as chlorophyll or CDOM concentration, butmeasurements are susceptible to variation caused by biofouling, instrument design, sensor drift, operating environment, and calibration rigor. To collect high quality data, such sensors need frequent checking and regular calibration. In this study, a wide variety of both liquid and solid fluorescentmaterials were trialed to assess their suitability as reference standards for performance assessment of in situ fluorometers. Criteria used to evaluate the standards included the spectral excitation/emission responses of the materials relative to fluorescence sensors and to targeted ocean properties, the linearity of the fluorometer’s optical response with increasing concentration, stability and consistency, availability and ease of use, as well as cost. Findings are summarized as a series of recommended reference standards for sensors deployed on stationary and mobile platforms, to suit avariety of in situ coastal to ocean sensor configurations. Repeated determinations of chlorophyll scale factor using the recommended liquid standard, Fluorescein, achieved an accuracy of 2.5%. Repeatedmeasurements with the recommended solid standard, Plexiglas Satinice® plum 4H01 DC (polymethylmethacrylate), over an 18 day period varied from the mean value by 1.0% for chlorophyll sensors and 3.3% for CDOM sensors.
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Review of fluorescent standards for calibration of in situ fluorometers: Recommendationsapplied in coastal and ocean observing programsEarp, A.; Hanson, C.; Ralph, P.; Brando, V.; Allen, S.; Baird, M.; Clementson, L.; Daniel, P.; Dekker, A.; Fearns, Peter; Parslow, J.; Strutton, P.; Thompson, P.; Underwood, M.; Weeks, S.; Doblin, M. (2011)Fluorometers are widely used in ecosystem observing to monitor fluorescence signals from organic compounds, as well as to infer geophysical parameters such as chlorophyll or CDOM concentration, butmeasurements are susceptible ...
Calibration procedures and first data set of Southern Ocean chlorophyll a profiles collected by elephant seals equipped with a newly developed CTD-fluorescence tagsGuinet, C.; Xing, X.; Walker, E.; Monestiez, P.; Marchand, S.; Picard, B.; Jaud, T.; Authier, M.; Cotte, C.; Dragon, A.; Diamond, E.; Antoine, David; Lovell, P.; Blain, S.; D'Ortenzio, F.; Claustre, H. (2013)In-situ observation of the marine environment has traditionally relied on ship-based platforms. The obvious consequence is that physical and biogeochemical properties have been dramatically undersampled, especially in the ...
Phytoplankton Photophysiology Utilities: A Python Toolbox for the Standardization of Processing Active Chlorophyll-a Fluorescence DataRyan-Keogh, T.J.; Robinson, Charlotte (2021)The uptake and application of single turnover chlorophyll fluorometers to the study of phytoplankton ecosystem status and microbial functions has grown considerably in the last two decades. However, standardization of ...