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    Cytochemistry of leukocytes from the family Macropodidae

    Access Status
    Fulltext not available
    Authors
    Hulme-Moir, K.
    Clark, Phillip
    Date
    2012
    Type
    Journal Article
    
    Metadata
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    Citation
    Hulme-Moir, K. and Clark, P. 2012. Cytochemistry of leukocytes from the family Macropodidae. Comparative Clinical Pathology. 21 (2): pp. 121-126.
    Source Title
    Comparative Clinical Pathology
    DOI
    10.1007/s00580-010-1071-9
    ISSN
    1618-5641
    School
    P.O. Box 288, Daw Park, Adelaide
    URI
    http://hdl.handle.net/20.500.11937/49348
    Collection
    • Curtin Research Publications
    Abstract

    The cytochemical staining characteristics of leukocytes from four species of Macropodidae and one species of Potoroidae were studied to investigate the cellular composition of leukocytes within the Macropodidae family and determine markers that may be useful for identifying cell lineage. Blood smears from the tammar wallaby (Macropus eugenii), the western grey kangaroo (Macropus fuliginosis), the red kangaroo (Macropus rufus), quokka (Setonix brachyurus) and a potoroid species, the woylie (Bettongia pencillata) were examined following reaction for Sudan Black B (SBB), peroxidase (PER), chloracetate esterase (CAE), a-naphthyl butyrate esterase (NBE) and alkaline phosphatase (ALP). Similar to domestic animal species, the neutrophils and eosinophils of macropodid species stained for both SBB and PER while monocytes and lymphocytes showed little or no reaction. CAE and NBE, however, were not useful as markers for macropodid neutrophils and monocytes, respectively. Significant variation between species was seen in ALP content.

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