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    Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces

    250266.pdf (746.9Kb)
    Access Status
    Open access
    Authors
    Alvarez de Eulate, Eva
    O'Sullivan, S.
    Arrigan, Damien
    Date
    2017
    Collection
    • Curtin Research Publications
    Type
    Journal Article
    Metadata
    Show full item record
    Abstract

    The formation of cytochrome c oligomers was induced at liquid−gel and liquid−liquid interfaces via electroadsorption. At an optimum interfacial potential (Eads=0.975 V), the protein was accumulated at these soft interfaces. It was found that as the concentration of adsorbed protein increased, a single voltammetric peak evolved into double and triple peaks (tads=300 s). Analysis of the protein that accumulated at the interfaces by polyacrylamide gel electrophoresis indicated the presence of oligomeric species, corresponding to dimers (ca. 27 kD), trimers (ca. 35 kD), and even larger species (>250 kD) after prolonged electroadsorption (tads=2 h) at macro-scale soft interfaces. Accordingly, it was possible to electrochemically induce oligomerisation at these soft interfaces, which can be tuned through experimental factors such as interfacial potential difference, electroadsorption time, and bulk solution concentration. These results suggest the use of electrochemistry at soft interfaces as a strategy for the investigation of protein oligomerisation and its inhibition.

    Citation
    Alvarez de Eulate, E. and O'Sullivan, S. and Arrigan, D. 2017. Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces. ChemElectroChem. 4 (4): pp. 898–904.
    Source Title
    ChemElectroChem
    URI
    http://hdl.handle.net/20.500.11937/50558
    DOI
    10.1002/celc.201600851
    Department
    Nanochemistry Research Institute
    Remarks

    This is the peer reviewed version of the following article: Alvarez de Eulate, E. and O'Sullivan, S. and Arrigan, D. 2017. Electrochemically Induced Formation of Cytochrome c Oligomers at Soft Interfaces. ChemElectroChem. 4 (4): pp. 898–904, which has been published in final form at 10.1002/celc.201600851. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving at http://olabout.wiley.com/WileyCDA/Section/id-828039.html

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