Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Diversifying selection in the wheat stem rust fungus acts predominantly on pathogen-associated gene families and reveals candidate effectors

    Access Status
    Open access via publisher
    Authors
    Sperschneider, J.
    Ying, H.
    Dodds, P.
    Gardiner, D.
    Upadhyaya, N.
    Singh, Karam
    Manners, J.
    Taylor, J.
    Date
    2014
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Sperschneider, J. and Ying, H. and Dodds, P. and Gardiner, D. and Upadhyaya, N. and Singh, K. and Manners, J. et al. 2014. Diversifying selection in the wheat stem rust fungus acts predominantly on pathogen-associated gene families and reveals candidate effectors. Frontiers in Plant Science. 5: 372.
    Source Title
    Frontiers in Plant Science
    DOI
    10.3389/fpls.2014.00372
    ISSN
    1664-462X
    School
    Centre for Crop Disease Management
    URI
    http://hdl.handle.net/20.500.11937/51690
    Collection
    • Curtin Research Publications
    Abstract

    Plant pathogens cause severe losses to crop plants and threaten global food production. One striking example is the wheat stem rust fungus, Puccinia graminis f. sp. tritici, which can rapidly evolve new virulent pathotypes in response to resistant host lines. Like several other filamentous fungal and oomycete plant pathogens, its genome features expanded gene families that have been implicated in host-pathogen interactions, possibly encoding effector proteins that interact directly with target host defense proteins. Previous efforts to understand virulence largely relied on the prediction of secreted, small and cysteine-rich proteins as candidate effectors and thus delivered an overwhelming number of candidates. Here, we implement an alternative analysis strategy that uses the signal of adaptive evolution as a line of evidence for effector function, combined with comparative information and expression data. We demonstrate that in planta up-regulated genes that are rapidly evolving are found almost exclusively in pathogen-associated gene families, affirming the impact of host-pathogen co-evolution on genome structure and the adaptive diversification of specialized gene families. In particular, we predict 42 effector candidates that are conserved only across pathogens, induced during infection and rapidly evolving. One of our top candidates has recently been shown to induce genotype-specific hypersensitive cell death in wheat. This shows that comparative genomics incorporating the evolutionary signal of adaptation is powerful for predicting effector candidates for laboratory verification. Our system can be applied to a wide range of pathogens and will give insight into host-pathogen dynamics, ultimately leading to progress in strategies for disease control.

    Related items

    Showing items related by title, author, creator and subject.

    • Comparative analysis of the predicted secretomes of Rosaceae scab pathogens Venturia inaequalis and V. pirina reveals expanded effector families and putative determinants of host range
      Deng, C.; Plummer, K.; Jones, Darcy; Mesarich, C.; Shiller, J.; Taranto, A.; Robinson, A.; Kastner, P.; Hall, N.; Templeton, M.; Bowen, J. (2017)
      © 2017 The New Zealand Institute for Plant and Food Research Limited. Background: Fungal plant pathogens belonging to the genus Venturia cause damaging scab diseases of members of the Rosaceae. In terms of economic impact, ...
    • Allelic barley MLA immune receptors recognize sequence-unrelated avirulence effectors of the powdery mildew pathogen
      Lu, X.; Kracher, B.; Saur, I.; Bauer, S.; Ellwood, Simon; Wise, R.; Yaeno, T.; Maekawa, T.; Schulze-Lefert, P. (2016)
      © 2016, National Academy of Sciences. All rights reserved. Disease-resistance genes encoding intracellular nucleotide-binding domain and leucine-rich repeat proteins (NLRs) are key components of the plant innate immune ...
    • Transcriptome analysis of the fungal pathogen Fusarium oxysporum f. sp. medicaginis during colonisation of resistant and susceptible Medicago truncatula hosts identifies differential pathogenicity profiles and novel candidate effectors
      Thatcher, L.; Williams, A.; Garg, G.; Buck, S.; Singh, Karam (2016)
      Background: Pathogenic members of the Fusarium oxysporum species complex are responsible for vascular wilt disease on many important crops including legumes, where they can be one of the most destructive disease causing ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.