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    Gelsolin-mediated activation of PI3K/Akt pathway is crucial for hepatocyte growth factor-induced cell scattering in gastric carcinoma

    Access Status
    Open access via publisher
    Authors
    Huang, B.
    Deng, S.
    Loo, S.
    Datta, A.
    Yap, Y.
    Yan, B.
    Ooi, C.
    Dinh, T.
    Zhuo, J.
    Tochhawng, L.
    Gopinadhan, S.
    Jegadeesan, T.
    Tan, P.
    Salto-Tellez, M.
    Yong, W.
    Soong, R.
    Yeoh, K.
    Goh, Y.
    Lobie, P.
    Yang, H.
    Kumar, Alan Prem
    Maciver, S.
    So, J.
    Yap, C.
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Huang, B. and Deng, S. and Loo, S. and Datta, A. and Yap, Y. and Yan, B. and Ooi, C. et al. 2016. Gelsolin-mediated activation of PI3K/Akt pathway is crucial for hepatocyte growth factor-induced cell scattering in gastric carcinoma. Oncotarget. 7 (18): pp. 25391-25407.
    Source Title
    Oncotarget
    DOI
    10.18632/oncotarget.8603
    ISSN
    1949-2553
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/51736
    Collection
    • Curtin Research Publications
    Abstract

    In gastric cancer (GC), the main subtypes (diffuse and intestinal types) differ in pathological characteristics, with diffuse GC exhibiting early disseminative and invasive behaviour. A distinctive feature of diffuse GC is loss of intercellular adhesion. Although widely attributed to mutations in the CDH1 gene encoding E-cadherin, a significant percentage of diffuse GC do not harbor CDH1 mutations. We found that the expression of the actin-modulating cytoskeletal protein, gelsolin, is significantly higher in diffuse-type compared to intestinal-type GCs, using immunohistochemical and microarray analysis. Furthermore, in GCs with wild-type CDH1, gelsolin expression correlated inversely with CDH1 gene expression. Downregulating gelsolin using siRNA in GC cells enhanced intercellular adhesion and E-cadherin expression, and reduced invasive capacity. Interestingly, hepatocyte growth factor (HGF) induced increasedgelsolin expression, and gelsolin was essential for HGF-medicated cell scattering and E-cadherin transcriptional repression through Snail, Twist and Zeb2. The HGF-dependent effect on E-cadherin was found to be mediated by interactions between gelsolin and PI3K-Akt signaling. This study reveals for the first time a function of gelsolin in the HGF/cMet oncogenic pathway, which leads to E-cadherin repression and cell scattering in gastric cancer. Our study highlights gelsolin as an important pro-disseminative factor contributing to the aggressive phenotype of diffuse GC.

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