Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Direct and Rapid Electrochemical Detection of Pseudomonas aeruginosa Quorum Signaling Molecules in Bacterial Cultures and Cystic Fibrosis Sputum Samples through Cationic Surfactant-Assisted Membrane Disruption

    Access Status
    Fulltext not available
    Authors
    Buzid, A.
    Reen, F.
    Langsi, V.
    Muimhneacháin, E.
    O'Gara, Fergal
    McGlacken, G.
    Luong, J.
    Glennon, J.
    Date
    2017
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Buzid, A. and Reen, F. and Langsi, V. and Muimhneacháin, E. and O'Gara, F. and McGlacken, G. and Luong, J. et al. 2017. Direct and Rapid Electrochemical Detection of Pseudomonas aeruginosa Quorum Signaling Molecules in Bacterial Cultures and Cystic Fibrosis Sputum Samples through Cationic Surfactant-Assisted Membrane Disruption. ChemElectroChem. 4 (3): pp. 533-541.
    Source Title
    ChemElectroChem
    DOI
    10.1002/celc.201600590
    ISSN
    2196-0216
    School
    School of Biomedical Sciences
    URI
    http://hdl.handle.net/20.500.11937/51981
    Collection
    • Curtin Research Publications
    Abstract

    Rapid detection of pathogenic bacteria present in patient samples is of utmost importance for the clinical management of bacterial-induced diseases. Herein, we describe an efficient and direct electrochemical approach for the detection of 2-heptyl-3-hydroxy-4-quinolone (PQS), 2-heptyl-4-hydroxyquinoline (HHQ), and pyocyanin (PYO) as a molecular signature of Pseudomonas aeruginosa (PA), a frequently infecting pathogen with high antibiotic resistance. The cationic surfactant hexadecyltrimethylammonium bromide (CTAB) enhances the effectiveness of an unmodified thin-film boron-doped diamond (BDD) electrode for the direct detection of PYO, HHQ, and PQS in bacterial cultures of PAO1 and PA14. Differential pulse voltammetry (DPV) is then used to monitor the production of these microbial metabolites in bacterial cultures of PAO1 over 10 h without any sample pretreatment. A proposed mechanism for the interaction of CTAB with bacteria cells is examined by zeta (?) potential measurements. Furthermore, the detection method is successfully extended to a clinical fluid matrix and applied to PA spiked cystic fibrosis (CF) sputum samples.

    Related items

    Showing items related by title, author, creator and subject.

    • Characterisation of aquatic natural organic matter by micro-scale sealed vessel pyrolysis
      Berwick, Lyndon (2009)
      The analytical capacity of MSSV pyrolysis has been used to extend the structural characterisation of aquatic natural organic matter (NOM). NOM can contribute to various potable water issues and is present in high ...
    • Comparison between Nasal Swabs and Nasopharyngeal Aspirates for, and Effect of Time in Transit on, Isolation of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, and Moraxella catarrhalis
      Carville, K.; Bowman, J.; Lehmann, Deborah; Riley, T. (2007)
      pmc logo image Logo of jcm Note: Performing your original search, comparison nasal swabs lehmann, in PubMed Central will retrieve 7 citations. Journal List > J Clin Microbiol > v.45(1); Jan 2007 Abstract ...
    • Inhibition of the endosymbiont “Candidatus Midichloria mitochondrii” during 16S rRNA gene profiling reveals potential pathogens in Ixodes ticks from Australia
      Gofton, A.; Oskam, C.; Lo, N.; Beninati, T.; Wei, H.; McCarl, V.; Murray, D.; Paparini, A.; Greay, T.; Holmes, A.; Bunce, Michael; Ryan, U.; Irwin, P. (2015)
      Background: The Australian paralysis tick (Ixodes holocyclus) is of significant medical and veterinary importance as a cause of dermatological and neurological disease, yet there is currently limited information about the ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.