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    Functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation

    Access Status
    Fulltext not available
    Authors
    Chooi, Y.
    Zhang, G.
    Hu, J.
    Muria Gonzalez, Jordi
    Tran, P.
    Pettitt, A.
    Maier, A.
    Barrow, R.
    Solomon, P.
    Date
    2017
    Type
    Journal Article
    
    Metadata
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    Citation
    Chooi, Y. and Zhang, G. and Hu, J. and Muria Gonzalez, J. and Tran, P. and Pettitt, A. and Maier, A. et al. 2017. Functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation. Environmental Microbiology.
    Source Title
    Environmental Microbiology
    DOI
    10.1111/1462-2920.13711
    ISSN
    1462-2912
    School
    Centre for Crop Disease Management
    URI
    http://hdl.handle.net/20.500.11937/52118
    Collection
    • Curtin Research Publications
    Abstract

    © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.Parastagonospora nodorum is an important pathogen of wheat. The contribution of secondary metabolites to this pathosystem is poorly understood. A biosynthetic gene cluster (SNOG_08608-08616) has been shown to be upregulated during the late stage of P. nodorum wheat leaf infection. The gene cluster shares several homologues with the Cercospora nicotianae CTB gene cluster encoding the biosynthesis of cercosporin. Activation of the gene cluster by overexpression (OE) of the transcription factor gene (SNOG_08609) in P. nodorum resulted in the production of elsinochrome C, a perelyenequinone phytotoxin structurally similar to cercosporin. Heterologous expression of the polyketide synthase gene elcA from the gene cluster in Aspergillus nidulans resulted in the production of the polyketide precursor nortoralactone common to the cercosporin pathway. Elsinochrome C could be detected on wheat leaves infected with P. nodorum, but not in the elcA disruption mutant. The compound was shown to exhibit necrotic activity on wheat leaves in a light-dependent manner. Wheat seedling infection assays showed that ?elcA exhibited reduced virulence compared with wild type, while infection by an OE strain overproducing elsinochrome C resulted in larger lesions on leaves. These data provided evidence that elsinochrome C contributes to the virulence of P. nodorum against wheat.

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