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    Subcellular tracking reveals the location of dimethylsulfoniopropionate in microalgae and visualises its uptake by marine bacteria

    Access Status
    Open access via publisher
    Authors
    Raina, J.
    Clode, P.
    Cheong, S.
    Bougoure, J.
    Kilburn, M.
    Reeder, A.
    Forêt, S.
    Stat, Michael
    Beltran, V.
    Thomas-Hall, P.
    Tapiolas, D.
    Motti, C.
    Gong, B.
    Pernice, M.
    Marjo, C.
    Seymour, J.
    Willis, B.
    Bourne, D.
    Date
    2017
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Raina, J. and Clode, P. and Cheong, S. and Bougoure, J. and Kilburn, M. and Reeder, A. and Forêt, S. et al. 2017. Subcellular tracking reveals the location of dimethylsulfoniopropionate in microalgae and visualises its uptake by marine bacteria. eLife. 6.
    Source Title
    eLife
    DOI
    10.7554/eLife.23008
    ISSN
    2050-084X
    School
    Department of Environment and Agriculture
    URI
    http://hdl.handle.net/20.500.11937/52840
    Collection
    • Curtin Research Publications
    Abstract

    © Raina et al.Phytoplankton-bacteria interactions drive the surface ocean sulfur cycle and local climatic processes through the production and exchange of a key compound: dimethylsulfoniopropionate (DMSP). Despite their large-scale implications, these interactions remain unquantified at the cellular-scale. Here we use secondary-ion mass spectrometry to provide the first visualization of DMSP at sub-cellular levels, tracking the fate of a stable sulfur isotope (34S) from its incorporation by microalgae as inorganic sulfate to its biosynthesis and exudation as DMSP, and finally its uptake and degradation by bacteria. Our results identify for the first time the storage locations of DMSP in microalgae, with high enrichments present in vacuoles, cytoplasm and chloroplasts. In addition, we quantify DMSP incorporation at the single-cell level, with DMSPdegrading bacteria containing seven times more 34S than the control strain. This study provides an unprecedented methodology to label, retain, and image small diffusible molecules, which can be transposable to other symbiotic systems.

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