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    Isolation and characterization of eight microsatellite loci in Chaetodon ornatissimus and cross-amplification in a sympatric sister species, Chaetodon meyeri

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    Authors
    Di Battista, Joseph
    Feldheim, K.
    Date
    2011
    Type
    Journal Article
    
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    Citation
    Di Battista, J. and Feldheim, K. 2011. Isolation and characterization of eight microsatellite loci in Chaetodon ornatissimus and cross-amplification in a sympatric sister species, Chaetodon meyeri. Conservation Genetics Resources. 3 (2): pp. 213-216.
    Source Title
    Conservation Genetics Resources
    DOI
    10.1007/s12686-010-9325-z
    ISSN
    1877-7252
    School
    Department of Environment and Agriculture
    URI
    http://hdl.handle.net/20.500.11937/53114
    Collection
    • Curtin Research Publications
    Abstract

    Eight polymorphic microsatellite loci were developed for sister species, Ornate butterflyfish (Chaetodon ornatissimus) and Scrawled butterflyfish (Chaetodon meyeri). All loci were scored from samples collected at geographically distinct locations (C. ornatissimus, N = 37, Republic of Palau; C. meyeri, N = 30, Republic of Kiribati). There were 14.00 or 13.50 mean alleles per locus (A) for C. ornatissimus and C. meyeri, respectively, with observed heterozygosity (H O) ranging from 0.61 to 0.97 and 0.60 to 1.00. Most loci conformed to Hardy-Weinberg expectations, were in linkage equilibrium, and lacked confounding null alleles. We expect these markers to be useful for resolving population structure and patterns of hybridization. © Springer Science+Business Media B.V. 2010.

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