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    Microsatellite DNA markers to resolve population structure and hybridization of two closely related surgeonfish species, Acanthurus nigricans and Acanthurus leucosternon

    Access Status
    Fulltext not available
    Authors
    Di Battista, Joseph
    Feldheim, K.
    Bowen, B.
    Date
    2011
    Type
    Journal Article
    
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    Citation
    Di Battista, J. and Feldheim, K. and Bowen, B. 2011. Microsatellite DNA markers to resolve population structure and hybridization of two closely related surgeonfish species, Acanthurus nigricans and Acanthurus leucosternon. Conservation Genetics Resources. 3 (1): pp. 159-162.
    Source Title
    Conservation Genetics Resources
    DOI
    10.1007/s12686-010-9313-3
    ISSN
    1877-7252
    School
    Department of Environment and Agriculture
    URI
    http://hdl.handle.net/20.500.11937/53175
    Collection
    • Curtin Research Publications
    Abstract

    Ten polymorphic microsatellite loci were developed for sister surgeonfish species: the white cheek surgeonfish (Acanthurus nigricans) which occurs primarily in the Pacific Ocean, and the powder blue tang (Acanthurus leucosternon) in the Indian Ocean. All loci were scored for widely separated collections (A. nigricans, N = 30, French Polynesia; A. leucosternon, N = 27, Republic of Seychelles; hybrids, N = 17, Cocos/Keeling Island, eastern Indian Ocean). For A. nigricans, A. leucosternon, and hybrids, respectively, the mean number of alleles per locus is A = 17.80, A = 16.60, and A = 14.60, while observed heterozygosity ranges from H O = 0.78-1.00, H O = 0.74-0.96, and H O = 0.69-1.00. With the exception of locus Ahy54, all loci conformed to Hardy-Weinberg expectations, most loci were in linkage equilibrium, and there was little evidence for confounding null alleles. These markers will be used to resolve range-wide population structure and patterns of hybridization.

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