Microsatellite DNA markers to resolve population structure and hybridization of two closely related surgeonfish species, Acanthurus nigricans and Acanthurus leucosternon
MetadataShow full item record
Ten polymorphic microsatellite loci were developed for sister surgeonfish species: the white cheek surgeonfish (Acanthurus nigricans) which occurs primarily in the Pacific Ocean, and the powder blue tang (Acanthurus leucosternon) in the Indian Ocean. All loci were scored for widely separated collections (A. nigricans, N = 30, French Polynesia; A. leucosternon, N = 27, Republic of Seychelles; hybrids, N = 17, Cocos/Keeling Island, eastern Indian Ocean). For A. nigricans, A. leucosternon, and hybrids, respectively, the mean number of alleles per locus is A = 17.80, A = 16.60, and A = 14.60, while observed heterozygosity ranges from H O = 0.78-1.00, H O = 0.74-0.96, and H O = 0.69-1.00. With the exception of locus Ahy54, all loci conformed to Hardy-Weinberg expectations, most loci were in linkage equilibrium, and there was little evidence for confounding null alleles. These markers will be used to resolve range-wide population structure and patterns of hybridization.
Showing items related by title, author, creator and subject.
Montanari, S.; van Herwerden, L.; Pratchett, M.; Hobbs, Jean-Paul; Fugedi, A. (2012)Natural hybridization is widespread among coral reef fishes. However, the ecological promoters and evolutionary consequences of reef fish hybridization have not been thoroughly evaluated. Butterflyfishes form a high number ...
Isolation and characterization of twenty microsatellite markers for the study of hybridization in butterflyfish of the genus ChaetodonMontanari, S.; Gardener, M.; Hobbs, Jean-Paul; Prachett, M.; Bay, L.; Van Herwerden, L. (2013)Twenty polymorphic microsatellite loci were developed via 454 sequencing for two hybridizing sister species of butterflyfish: the spot-band butterflyfish (Chaetodon punctatofasciatus) and peppered butterflyfish (Chaetodon ...
Gene-enriched draft genome of the cattle tick Rhipicephalus microplus: Assembly by the hybrid Pacific Biosciences/Illumina approach enabled analysis of the highly repetitive genomeBarrero, R.; Guerrero, F.; Black, M.; McCooke, J.; Chapman, B.; Schilkey, F.; Pérez de León, A.; Miller, R.; Bruns, S.; Dobry, J.; Mikhaylenko, G.; Stormo, K.; Bell, C.; Tao, Q.; Bogden, R.; Moolhuijzen, Paula; Hunter, A.; Bellgard, M. (2016)The genome of the cattle tick Rhipicephalus microplus, an ectoparasite with global distribution, is estimated to be 7.1. Gbp in length and consists of approximately 70% repetitive DNA. We report the draft assembly of a ...