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dc.contributor.authorNguyen, D.
dc.contributor.authorJohnson, Stuart
dc.contributor.authorClarke, M.
dc.date.accessioned2017-06-23T03:01:46Z
dc.date.available2017-06-23T03:01:46Z
dc.date.created2017-06-19T03:39:29Z
dc.date.issued2017
dc.identifier.citationNguyen, D. and Johnson, S. and Clarke, M. 2017. Identification and Quantification of Dityrosine in Grain Proteins by Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry. Food Analytical Methods. 10 (10): pp. 3321-3328.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/53876
dc.identifier.doi10.1007/s12161-017-0901-7
dc.description.abstract

A novel liquid chromatography-tandem mass spectrometry method was developed and validated for identification and quantification of dityrosine in zein protein. The zein samples spiked with a dityrosine standard and 3,3-13C6-dityrosine, as the internal standard were hydrolysed in a mixture of 6 N hydrochloric acid and propionic acid followed by cleaning-up using Sep Pak C18 cartridges. Good linear regression (R2 = 0.999) was obtained in the range 1–1000 ng/mL. Method limit of detection and method limit of quantification were 42.1 and 140 ng/g, respectively. Recoveries were from 92 to 95.2%. Precision and inter-day reproducibility expressed as relative standard deviation were from 3.9 to 22.1% and from 4.4 to 16.6%, respectively. The validated method was applied to quantify dityrosine in various grain proteins. The levels of dityrosine ranged from 0.38 to 1.92 ng/mg. This validated method will be of great value for understanding the role of dityrosine in grain food texture.

dc.titleIdentification and Quantification of Dityrosine in Grain Proteins by Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry
dc.typeJournal Article
dcterms.source.startPage1
dcterms.source.endPage8
dcterms.source.issn1936-9751
dcterms.source.titleFood Analytical Methods
curtin.departmentSchool of Public Health
curtin.accessStatusFulltext not available


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