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    Otitis-prone children produce functional antibodies to pneumolysin and pneumococcal polysaccharides

    Access Status
    Open access via publisher
    Authors
    Kirkham, L.
    Wiertsema, S.
    Corscadden, K.
    Mateus, T.
    Mullaney, G.
    Zhang, Guicheng
    Richmond, P.
    Thornton, R.
    Date
    2017
    Type
    Conference Paper
    
    Metadata
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    Citation
    Kirkham, L. and Wiertsema, S. and Corscadden, K. and Mateus, T. and Mullaney, G. and Zhang, G. and Richmond, P. et al. 2017. Otitis-prone children produce functional antibodies to pneumolysin and pneumococcal polysaccharides, International Congress of Immunology (ICI), pp. 641-641.
    Source Title
    Clinical and Vaccine Immunology
    Source Conference
    International Congress of Immunology (ICI)
    DOI
    10.1128/CVI.00497-16
    ISSN
    1556-6811
    School
    School of Public Health
    URI
    http://hdl.handle.net/20.500.11937/54325
    Collection
    • Curtin Research Publications
    Abstract

    Copyright © 2017 Kirkham et al. The pneumococcus is a major otitis media (OM) pathogen, but data are conflicting regarding whether otitis-prone children have impaired humoral immunity to pneumococcal antigens. We and others have shown that otitis-prone and healthy children have similar antibody titers to pneumococcal proteins and polysaccharides (vaccine and nonvaccine types); however, the quality of antibodies from otitis-prone children has not been investigated. Antibody function, rather than titer, is considered to be a better correlate of protection from pneumococcal disease. Therefore, we compared the capacities of antibodies from otitis-prone (cases) and healthy (controls) children to neutralize pneumolysin, the pneumococcal toxin currently in development as a vaccine antigen, and to opsonize pneumococcal vaccine and nonvaccine serotypes. A pneumolysin neutralization assay was conducted on cholesterol-depleted complementinactivated sera from 165 cases and 61 controls. A multiplex opsonophagocytosis assay (MOPA) was conducted on sera from 20 cases and 20 controls. Neutralizing and opsonizing titers were calculated with antigen-specific IgG titers to determine antibody potency for pneumolysin, pneumococcal conjugate vaccine (PCV) polysaccharides, and non-PCV polysaccharides. There was no significant difference in antibody potencies between cases and controls for the antigens tested. Antipneumolysin neutralizing titers increased with the number of episodes of acute OM, but antibody potency did not. Pneumolysin antibody potency was lower in children colonized with pneumococci than in noncarriers, and this was significant for the otitis-prone group (P < 0.05). The production of functional antipneumococcal antibodies in otitisprone children demonstrates that they respond to the current PCV and are likely to respond to pneumolysin-based vaccines as effectively as healthy children.

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