Small macrophages are present in early childhood respiratory disease
MetadataShow full item record
Background: Recently, an established "small macrophage" phenotype has been observed in the sputum of patients with CF and COPD. However, little is known about the prevalence of this phenotype in the airways of young children. Since respiratory inflammation begins early in CF, we hypothesised that these small macrophages would be increased in paediatric CF bronchoalveolar lavage (BAL). Methods: Macrophage populations in CF and disease control BAL were assessed by multicolour flow cytometry. BAL inflammatory indices were collected as part of the AREST-CF programme. Results: Small macrophages were present in CF (n = 35, mean 36 ± 12% of BAL macrophages) but not significantly different to the respiratory disease controls (n = 7, mean 40 ± 21%). Number of small macrophages correlated significantly with number of BAL neutrophils (r = 0.44, p< 0.01) but not infection or IL-8. Conclusions: In paediatric patients small macrophages are not unique to CF, but their establishment as the dominant phenotype in adults may be due to chronicity of inflammation and infection.
Showing items related by title, author, creator and subject.
Jackaman, Connie; Radley-Crabb, Hannah; Soffe, Z.; Shavlakadze, T.; Grounds, M.; Nelson, Delia (2013)Changes to innate cells, such as macrophages and myeloid-derived suppressor cells (MDSCs), during aging in healthy or tumor-bearing hosts are not well understood. We compared macrophage subpopulations and MDSCs from healthy ...
Inhibition of destructive autoimmune arthritis in Fc?RIIa transgenic mice by small chemical entitiesPietersz, G.; Mottram, P.; Van De Velde, N.; Sardjono, C.; Esparon, S.; Ramsland, Paul; Moloney, G.; Baell, J.; McCarthy, T.; Matthews, B.; Powell, M.; Hogarth, P. (2009)The interaction of immune complexes with the human Fc receptor, Fc?RIIa, initiates the release of inflammatory mediators and is implicated in the pathogenesis of human autoimmune diseases, including rheumatoid arthritis ...
ADAM28 is elevated in humans with the metabolic syndrome and is a novel sheddase of human tumour necrosis factor-aJowett, J.; Okada, Y.; Leedman, P.; Curran, J.; Johnson, M.; Moses, Eric; Goring, H.; Mochizuki, S.; Blangero, J.; Stone, L.; Allen, H.; Mitchell, C.; Matthews, V. (2012)Metalloproteinases are implicated in cleaving numerous proinflammatory mediators from the cell surface. Interestingly, the elevated levels of tumour necrosis factor-α (TNF-α) have been associated with the metabolic syndrome. ...