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    Rare pyrenophora teres hybridization events revealed by development of sequence-specific PCR markers

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    Fulltext not available
    Authors
    Poudel, B.
    Ellwood, Simon
    Testa, A.
    McLean, M.
    Sutherland, M.
    Martin, A.
    Date
    2017
    Type
    Journal Article
    
    Metadata
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    Citation
    Poudel, B. and Ellwood, S. and Testa, A. and McLean, M. and Sutherland, M. and Martin, A. 2017. Rare pyrenophora teres hybridization events revealed by development of sequence-specific PCR markers. Phytopathology. 107 (7): pp. 878-884.
    Source Title
    Phytopathology
    DOI
    10.1094/PHYTO-11-16-0396-R
    ISSN
    0031-949X
    School
    Centre for Crop Disease Management
    URI
    http://hdl.handle.net/20.500.11937/54684
    Collection
    • Curtin Research Publications
    Abstract

    Pyrenophora teres f. teres and P. teres f. Maculata cause net form and spot form, respectively, of net blotch on barley (Hordeum vulgare). The two forms reproduce sexually, producing hybrids with genetic and pathogenic variability. Phenotypic identification of hybrids is challenging because lesions induced by hybrids on host plants resemble lesions induced by either P. teres f. teres or P. teres f. Maculata. In this study, 12 sequence-specific polymerase chain reaction markers were developed based on expressed regions spread across the genome. The primers were validated using 210 P. teres isolates, 2 putative field hybrids (WAC10721 and SNB172), 50 laboratory-produced hybrids, and 7 isolates collected from barley grass (H. leporinum). The sequence-specific markers confirmed isolate WAC10721 as a hybrid. Only four P. teres f. teres markers amplified on DNA of barley grass isolates. Amplified fragment length polymorphism markers suggested that P. teres barley grass isolates are genetically different from P. teres barley isolates and that the second putative hybrid (SNB172) is a barley grass isolate. We developed a suite of markers which clearly distinguish the two forms of P. teres and enable unambiguous identification of hybrids.

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