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A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery

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Authors
Gu, Y.
Guo, Y.
Wang, C.
Xu, J.
Wu, Jian-Ping
Kirk, T.
Ma, D.
Xue, W.
Date
2017
Type
Journal Article

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Gu, Y. and Guo, Y. and Wang, C. and Xu, J. and Wu, J. and Kirk, T. and Ma, D. et al. 2017. A polyamidoamne dendrimer functionalized graphene oxide for DOX and MMP-9 shRNA plasmid co-delivery. Materials Science and Engineering C. 70: pp. 572-585.
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Materials Science and Engineering C
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Department of Mechanical Engineering
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Abstract

© 2016 Elsevier B.V. It is a promising way to treat the multi drug resistance (MDR) of tumor cells in both of drug and gene methods. A polyamidoamne dendrimer functionalized graphene oxide (GO-PAMAM) was designed, which could load doxorubicin (DOX) and MMP-9 shRNA plasmid at the same time in order to achieve effective treatment to breast cancer. GO-PAMAM has a high loading capacity to DOX and pH-controlled DOX release. Besides, it has efficient gene transfer ability, the transfection efficiency is significantly better than PEI-25k in the presence of serum, and it can significantly inhibit the expression of MMP-9 protein in MCF-7 cells. The effect of DOX and MMP-9 shRNA plasmid co-delivery was more significant than that of the single drug. Moreover, GO-PAMAM exhibited lower cytotoxicity compared to PEI-25k in CCK-8 assays, and also showed a good biocompatibility in vivo. Therefore, GO-PAMAM will have broad prospects for drug and gene co-delivery.