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dc.contributor.authorLeali, D.
dc.contributor.authorInforzato, A.
dc.contributor.authorRonca, R.
dc.contributor.authorBianchi, R.
dc.contributor.authorBelleri, M.
dc.contributor.authorColtrini, D.
dc.contributor.authorDi Salle, E.
dc.contributor.authorSironi, M.
dc.contributor.authorNorata, Giuseppe
dc.contributor.authorBottazzi, B.
dc.contributor.authorGarlanda, C.
dc.contributor.authorDay, A.
dc.contributor.authorPresta, M.
dc.identifier.citationLeali, D. and Inforzato, A. and Ronca, R. and Bianchi, R. and Belleri, M. and Coltrini, D. and Di Salle, E. et al. 2012. Long pentraxin 3/tumor necrosis factor-stimulated gene-6 interaction a biological rheostat for fibroblast growth factor 2-mediated angiogenesis. Arteriosclerosis, Thrombosis, and Vascular Biology. 32 (3): pp. 696-703.

Objective-Angiogenesis is regulated by the balance between pro-and antiangiogenic factors and by extracellular matrix protein interactions. Fibroblast growth factor 2 (FGF2) is a major proangiogenic inducer inhibited by the interaction with the soluble pattern recognition receptor long pentraxin 3 (PTX3). PTX3 is locally coexpressed with its ligand tumor necrosis factor-stimulated gene-6 (TSG-6), a secreted glycoprotein that cooperates with PTX3 in extracellular matrix assembly. Here, we characterized the effect of TSG-6 on PTX3/FGF2 interaction and FGF2-mediated angiogenesis. Methods and Results-Solid phase binding and surface plasmon resonance assays show that TSG-6 and FGF2 bind the PTX3 N-terminal domain with similar affinity. Accordingly, TSG-6 prevents FGF2/PTX3 interaction and suppresses the inhibition exerted by PTX3 on heparan sulfate proteoglycan/FGF2/FGF receptor complex formation and on FGF2-dependent angiogenesis in vitro and in vivo. Also, endogenous PTX3 exerts an inhibitory effect on vascularization induced by FGF2 in a murine subcutaneous Matrigel plug assay, the inhibition being abolished in Ptx3-null mice or by TSG-6 treatment in wild-type animals. Conclusion-TSG-6 reverts the inhibitory effects exerted by PTX3 on FGF2-mediated angiogenesis through competition of FGF2/PTX3 interaction. This may provide a novel mechanism to control angiogenesis in those pathological settings characterized by the coexpression of TSG-6 and PTX3, in which the relative levels of these proteins may fine-tune the angiogenic activity of FGF2. © 2012 American Heart Association, Inc.

dc.publisherLippincott Williams & Wilkins
dc.titleLong pentraxin 3/tumor necrosis factor-stimulated gene-6 interaction a biological rheostat for fibroblast growth factor 2-mediated angiogenesis
dc.typeJournal Article
dcterms.source.titleArteriosclerosis, Thrombosis, and Vascular Biology
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusOpen access via publisher

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