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    Identification of sources of resistance to Phoma medicaginis isolates in Medicago truncatula SARDI core collection accessions, and multigene differentiation of isolates

    Access Status
    Fulltext not available
    Authors
    Ellwood, Simon
    Kamphuis, L.
    Oliver, Richard
    Date
    2006
    Type
    Journal Article
    
    Metadata
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    Citation
    ELLWOOD SR, KAMPHUIS LG.& OLIVER RP.(2006) Identification of sources of resistance to Phoma medicaginis isolates in Medicago truncatula SARDI core collection accessions, and multigene differentiation of isolates. Phytopathology 96 1330-1336
    DOI
    10.1094/PHYTO-96-1330
    Faculty
    Department of Environmental & Agriculture
    School of Agriculture and Environment
    Faculty of Science and Engineering
    Remarks

    A copy of this item may be available from Professor Richard Oliver

    Email: Richard.oliver@curtin.edu.au

    URI
    http://hdl.handle.net/20.500.11937/5577
    Collection
    • Curtin Research Publications
    Abstract

    Phoma medicaginis is a necrotrophic fungal pathogen, commonly found infecting the annual medic Medicago truncatula. To differentiate eight P. medicaginis isolates, five gene regions were examined: actin, β-tubulin, calmodulin, translation elongation factor 1-α (EF-1α), and the internal transcribed spacer ribosomal DNA. Sequence comparisons showed that specimens isolated from M. truncatula in Western Australia formed a group that was consistently different from, but allied to, a P. medicaginis var. medicaginis type specimen. EF-1α contained a hyper-variable 55-bp repeat unit, which forms the basis of a rapid polymerase chain reaction-based method of reliably distinguishing isolates. Characterization of three isolates showed that all exhibited a narrow host range, causing disease only in M. sativa and M. truncatula among eight commonly cultivated legume species sampled. Infection of 86 M. truncatula single-seeded accessions showed a continuous distribution in disease phenotypes, with the majority of accessions susceptible. On a 1-to-5 disease reaction scale increasing in severity, individual fungal isolates showed means of 2.6 to 3.2, and scores ranged from 1 to 4.8 among accessions. The results presented here suggest that M. truncatula harbors specific and diverse sources of resistance to individual P. medicaginis genotypes.

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