Effector memory T cells are associated with atherosclerosis in humans and animal models
|dc.contributor.author||De Metrio, M.|
|dc.identifier.citation||Ammirati, E. and Cianflone, D. and Vecchio, V. and Banfi, M. and Vermi, A. and De Metrio, M. and Grigore, L. et al. 2012. Effector memory T cells are associated with atherosclerosis in humans and animal models. Journal of the American Heart Association. 1 (1): pp. 27-41.|
Background- Adaptive T-cell response is promoted during atherogenesis and results in the differentiation of näive CD4 + T cells to effector and/or memory cells of specialized T-cell subsets. Aim of this work was to investigate the relationship between circulating CD4 + T-cell subsets and atherosclerosis. Methods and Results- We analyzed 57 subsets of circulating CD4+T cells by 10-parameter/8-color polychromatic flow cytometry (markers: CD3/CD4/CD45RO/CD45RA/CCR7/CCR5/CXCR3/HLA-DR) in peripheral blood from 313 subjects derived from 2 independent cohorts. In the first cohort of subjects from a free-living population (n=183), effector memory T cells (T EM : CD3 + CD4 + CD45RA - CD45RO + CCR7- cells) were strongly related with intima-media thickness of the common carotid artery, even after adjustment for age (r=0.27; P < 0.001). Of note, a significant correlation between T EM and low-density lipoproteins was observed. In the second cohort (n=130), T EM levels were significantly increased in patients with chronic stable angina or acute myocardial infarction compared with controls. HLA-DR+T EM were the T EM subpopulation with the strongest association with the atherosclerotic process (r=0.37; P < 0.01). Finally, in animal models of atherosclerosis, T EM (identified as CD4 + CD44 + CD62L - ) were significantly increased in low-density lipoprotein receptor and apolipoprotein E deficient mice compared with controls and were correlated with the extent of atherosclerotic lesions in the aortic root (r=0.56; P < 0.01). Conclusions- Circulating T EM cells are associated with increased atherosclerosis and coronary artery disease in humans and in animal models and could represent a key CD4 + T-cell subset related to the atherosclerotic process. © 2012. The Authors.
|dc.title||Effector memory T cells are associated with atherosclerosis in humans and animal models|
|dcterms.source.title||Journal of the American Heart Association|
|curtin.department||School of Biomedical Sciences|
|curtin.accessStatus||Open access via publisher|
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