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    Isolation of Thermophilic Lignin Degrading Bacteria from Oil-Palm Empty Fruit Bunch (EFB) Compost

    Access Status
    Open access via publisher
    Authors
    Lai, C.
    Chua, H.
    Danquah, M.
    Saptoro, Agus
    Date
    2017
    Type
    Conference Paper
    
    Metadata
    Show full item record
    Citation
    Lai, C. and Chua, H. and Danquah, M. and Saptoro, A. 2017. Isolation of Thermophilic Lignin Degrading Bacteria from Oil-Palm Empty Fruit Bunch (EFB) Compost. IOP Materials Science and Engineering. 206: 012016.
    Source Title
    IOP Materials Science and Engineering
    DOI
    10.1088/1757-899X/206/1/012016
    ISSN
    1757-8981
    School
    Curtin Sarawak
    URI
    http://hdl.handle.net/20.500.11937/55870
    Collection
    • Curtin Research Publications
    Abstract

    © Published under licence by IOP Publishing Ltd. Empty Fruit Bunch (EFB) is a potential and sustainable feedstock for bioethanol production due to its high cellulosic content and availability in Malaysia. Due to high lignin content of EFB and the lack of effective delignification process, commercial bioethanol production from EFB is presently not viable. Enzymatic delignification has been identified as one of the key steps in utilising EFB as a feedstock for bioethanol conversion. To date, limited work has been reported on the isolation of lignin degrading bacteria. Hence, there is a growing interest to search for new lignin degrading bacteria with greater tolerance to temperature and high level of ligninolytic enzymes for more effective lignin degradation. This study aimed to isolate and screen thermophilic ligninolytic microorganisms from EFB compost. Ten isolates were successfully isolated from EFB compost. Although they are not capable of decolorizing Methylene Blue (MB) dye under agar plate assay method, they are able to utilize lignin mimicked compound - guaiacol as a sole carbon on the agar plate assay. This infers that there is no correlation of ligninolytic enzymes with dye decolourization for all the isolates that have been isolated. However, they are able to produce ligninolytic enzymes (Lignin peroxidase, Manganese peroxidase, Laccase) in Minimal Salt Medium with Kraft Lignin (MSM-KL) with Lignin Peroxidase (LiP) as the predominant enzyme followed by Manganese Peroxidase (MnP) and Laccase (Lac). Among all the tested isolates, CLMT 29 has the highest LiP production up to 8.7673 U/mL following 24 h of growth.

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