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dc.contributor.authorHadden, W.
dc.contributor.authorYoung, J.
dc.contributor.authorHolle, A.
dc.contributor.authorMcFetridge, M.
dc.contributor.authorKim, Du Yong
dc.contributor.authorWijesinghe, P.
dc.contributor.authorTaylor-Weiner, H.
dc.contributor.authorWen, J.
dc.contributor.authorLee, A.
dc.contributor.authorBieback, K.
dc.contributor.authorVo, Ba-Ngu
dc.contributor.authorSampson, D.
dc.contributor.authorKennedy, B.
dc.contributor.authorSpatz, J.
dc.contributor.authorEngler, A.
dc.contributor.authorCho, Y.
dc.date.accessioned2017-08-24T02:22:19Z
dc.date.available2017-08-24T02:22:19Z
dc.date.created2017-08-23T07:21:43Z
dc.date.issued2017
dc.identifier.citationHadden, W. and Young, J. and Holle, A. and McFetridge, M. and Kim, D.Y. and Wijesinghe, P. and Taylor-Weiner, H. et al. 2017. Stem cell migration and mechanotransduction on linear stiffness gradient hydrogels. Proceedings of the National Academy of Sciences of USA. 114 (22): pp. 5647-5652.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/56114
dc.identifier.doi10.1073/pnas.1618239114
dc.description.abstract

The spatial presentation of mechanical information is a key parameter for cell behavior. We have developed a method of polymerization control in which the differential diffusion distance of unreacted cross-linker and monomer into a prepolymerized hydrogel sink results in a tunable stiffness gradient at the cell-matrix interface. This simple, low-cost, robust method was used to produce polyacrylamide hydrogels with stiffness gradients of 0.5, 1.7, 2.9, 4.5, 6.8, and 8.2 kPa/mm, spanning the in vivo physiological and pathological mechanical landscape. Importantly, three of these gradients were found to be nondurotactic for human adipose-derived stem cells (hASCs), allowing the presentation of a continuous range of stiffnesses in a single well without the confounding effect of differential cell migration. Using these nondurotactic gradient gels, stiffness-dependent hASC morphology, migration, and differentiation were studied. Finally, the mechanosensitive proteins YAP, Lamin A/C, Lamin B, MRTF-A, and MRTF-B were analyzed on these gradients, providing higher-resolution data on stiffness-dependent expression and localization.

dc.publisherNational Academy of Sciences
dc.titleStem cell migration and mechanotransduction on linear stiffness gradient hydrogels
dc.typeJournal Article
dcterms.source.volume114
dcterms.source.number22
dcterms.source.startPage5647
dcterms.source.endPage5652
dcterms.source.issn0027-8424
dcterms.source.titleProceedings of the National Academy of Sciences of USA
curtin.departmentDepartment of Electrical and Computer Engineering
curtin.accessStatusOpen access via publisher


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