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    Human methicillin-sensitive Staphylococcus aureus biofilms: potential associations with antibiotic resistance persistence and surface polysaccharide antigens

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    Fulltext not available
    Authors
    Babra, C.
    Gogoi Tiwari, Jully
    Costantino, Paul
    Sunagar, R.
    Isloor, S.
    Hegde, N.
    Mukkur, Trilochan
    Date
    2013
    Type
    Journal Article
    
    Metadata
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    Citation
    Babra, Charlene and Gogoi Tiwari, Jully and Costantino, Paul and Sunagar, Raju and Isloor, Shrikrishna and Hegde, Nagendra and Mukkur, Trilochan. 2013. Human methicillin-sensitive Staphylococcus aureus biofilms: potential associations with antibiotic resistance persistence and surface polysaccharide antigens. Journal of Basic Microbiology. 54 (7): pp. 721-728.
    Source Title
    Journal of Basic Microbiology
    DOI
    10.1002/jobm.201200557
    ISSN
    0233-111X
    URI
    http://hdl.handle.net/20.500.11937/5728
    Collection
    • Curtin Research Publications
    Abstract

    The development of persistent antibiotic resistance by human methicillin-sensitive Staphylococcus aureus (MSSA) strains and substantial association with poly-N-acetyl glucosamine (PNAG) in biofilms is reported in this investigation. Sixteen of 31 MSSA strains under study were found to have developed resistance to one or more antibiotics, with four strains, two of which did not produce biofilms, showing resistance to cefoxitin, undetectable by mecA amplification. Antibiotic resistance displayed by 13/14 biofilm-forming S. aureus isolates remained persistent for 4 weeks prior to reverting back to the original antibiotic susceptibility, prompting a suggestion of determining antibiograms for clinical S. aureus isolates subcultured from biofilms developed in vitro as well as planktonic subcultures prepared from the site of infection. While there was correlation of antibiotic resistance with biofilm formation confirming previous reports, this is the first time that persistence of the biofilm-associated antibiotic resistance by S. aureus as planktonic cells is reported. Among the two methods used for assessment of biofilm formation, the tissue culture plate (TCP) method revealed that almost all strains were strong or moderate biofilm producers whereas only 19/31 strains were biofilm producers using the Congo Red agar (CRA) method indicating the superiority of the TCP method in detecting biofilm producers. We alsoobserved no association between biofilm formation and major capsule types. However, substantial, although not absolute, association of biofilm formation with PNAG was observed, warranting continued identification of additional surface-associated polysaccharide and/or protein antigens associated with biofilm formation for development of an effective vaccine against S. aureus infections regardless of capsular phenotype.

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