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    Monitoring of oxidative status in three native Australian species during cold acclimation and cryopreservation

    Access Status
    Fulltext not available
    Authors
    Funnekotter, Bryn
    Colville, L.
    Kaczmarczyk, A.
    Turner, S.
    Bunn, E.
    Mancera, Ricardo
    Date
    2017
    Type
    Journal Article
    
    Metadata
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    Citation
    Funnekotter, B. and Colville, L. and Kaczmarczyk, A. and Turner, S. and Bunn, E. and Mancera, R. 2017. Monitoring of oxidative status in three native Australian species during cold acclimation and cryopreservation. Plant Cell Reports. 36 (12): pp. 1903-1916.
    Source Title
    Plant Cell Reports
    DOI
    10.1007/s00299-017-2204-2
    ISSN
    0721-7714
    School
    School of Biomedical Sciences
    Funding and Sponsorship
    http://purl.org/au-research/grants/arc/LP140100993
    URI
    http://hdl.handle.net/20.500.11937/58289
    Collection
    • Curtin Research Publications
    Abstract

    © 2017, Springer-Verlag GmbH Germany. Key message: Three wild species exhibited a significant reduction in antioxidants throughout the cryopreservation protocol, whilst the half-cell reduction potential became more oxidised. Antioxidant content recuperated in recovering shoot tips. Abstract: Cryopreservation is the most efficient and cost-effective long-term storage solution for the conservation of a wide range of plant species and material. Changes in the levels of antioxidants during the process of cryopreservation are known to reduce post-cryogenic survival due to oxidative stress. Low-molecular-weight thiols (cysteine, ?-glutamylcysteine, and glutathione) and ascorbic acid, which represent the two major water-soluble antioxidants in plants, were analysed at specific stages during cryopreservation of shoot tip material of three native Australian plant species [Anigozanthos viridis (Haemodoraceae), Lomandra sonderi (Asparagaceae), and Loxocarya cinerea (Restionaceae)] to quantify the oxidative stress experienced during cryopreservation. Post-cryogenic regeneration of shoot tips was greatest in A. viridis (78%) followed by L. sonderi (50%), whilst L. cinerea did not show any post-cryogenic regeneration. The application of a 3-week cold (5 °C) preconditioning regime, commonly used to increase post-cryogenic survival, resulted in significantly lower post-cryogenic regeneration for A. viridis (33%), but had little effect on the other two species. Total antioxidant concentration in shoot material decreased significantly with each step throughout the cryopreservation process, particularly in the cryoprotection and washing stages. Antioxidant levels in shoot tips then increased during the subsequent 7-day post-cryopreservation recovery period, with the greatest increase measured in A. viridis. Concentrations of thiols and their corresponding disulphides were used to calculate the corresponding half-cell reduction potentials, whereby the ability of these plant species to maintain a strong reducing environment in shoot tissues throughout the cryopreservation protocol was found to correlate with post-cryogenic survival.

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