Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Enabling dual cellular destinations of polymeric nanoparticles for treatment following partial injury to the central nervous system

    257348.pdf (4.205Mb)
    Access Status
    Open access
    Authors
    Lozic, I.
    Hartz, R.
    Bartlett, C.
    Shaw, J.
    Archer, M.
    Naidu, P.
    Smith, N.
    Dunlop, S.
    Iyer, K.
    Kilburn, M.
    Fitzgerald, Melinda
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Lozic, I. and Hartz, R. and Bartlett, C. and Shaw, J. and Archer, M. and Naidu, P. and Smith, N. et al. 2016. Enabling dual cellular destinations of polymeric nanoparticles for treatment following partial injury to the central nervous system. Biomaterials. 74: pp. 200-216.
    Source Title
    Biomaterials
    DOI
    10.1016/j.biomaterials.2015.10.001
    ISSN
    0142-9612
    School
    Health Sciences Research and Graduate Studies
    URI
    http://hdl.handle.net/20.500.11937/58803
    Collection
    • Curtin Research Publications
    Abstract

    Following neurotrauma, oxidative stress is spread via the astrocytic syncytium and is associated with increased aquaporin 4 (AQP4), inflammatory cell infiltration, loss of neurons and glia and functional deficits. Herein we evaluate multimodal polymeric nanoparticles functionalized with an antibody to an extracellular epitope of AQP4, for targeted delivery of an anti-oxidant as a therapeutic strategy following partial optic nerve transection. Using fluorescence microscopy, spectrophotometry, correlative nanoscale secondary ion mass spectrometry (NanoSIMS) and transmission electron microscopy, in vitro and in vivo, we demonstrate that functionalized nanoparticles are coated with serum proteins such as albumin and enter both macrophages and astrocytes when administered to the site of a partial optic nerve transection in rat. Antibody functionalized nanoparticles synthesized to deliver the antioxidant resveratrol are effective in reducing oxidative damage to DNA, AQP4 immunoreactivity and preserving visual function. Non-functionalized nanoparticles evade macrophages more effectively and are found more diffusely, including in astrocytes, however they do not preserve the optic nerve from oxidative damage or functional loss following injury. Our study highlights the need to comprehensively investigate nanoparticle location, interactions and effects, both in vitro and in vivo, in order to fully understand functional outcomes.

    Related items

    Showing items related by title, author, creator and subject.

    • Aqueous Eucalyptus globulus leaf extract-mediated biosynthesis of MgO nanorods
      Jeevanandam, J.; Chan, Stephanie; Ku, Y. (2018)
      Plant-based biosynthesis is gaining attention in nanoparticle synthesis as an alternate to chemical and physical synthesis routes due to their non-toxic and environment friendly nature. Leaf extract-based biosynthesis ...
    • Biosynthesis and characterization of MgO nanoparticles from plant extracts via induced molecular nucleation
      Jeevanandam, J.; Chan, Stephanie; Danquah, M. (2017)
      © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique. Plant based green synthesis approaches are gaining prominence due to their simplicity, cost-effectiveness, ecological friendliness, ...
    • Calcination-Dependent Morphology Transformation of Sol-Gel- Synthesized MgO Nanoparticles
      Jeevanandam, J.; Chan, Stephanie; Danquah, M. (2017)
      © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Sol-gel synthesis is a widely accepted method of producing nanoparticles with high surface area-to-volume ratio and purity at relatively low temperatures. For metal oxide ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.