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    The murine choline-deficient, ethionine-supplemented (CDE) diet model of chronic liver injury

    Access Status
    Fulltext not available
    Authors
    Gogoi-Tiwari, J.
    Köhn-Gaone, J.
    Giles, C.
    Schmidt-Arras, D.
    Gratte, F.
    Elsegood, Caryn
    McCaughan, G.
    Ramm, G.
    Olynyk, John
    Tirnitz-Parker, Nina
    Date
    2017
    Collection
    • Curtin Research Publications
    Type
    Journal Article
    Metadata
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    Abstract

    Chronic liver diseases, such as viral hepatitis, alcoholic liver disease, or non-alcoholic fatty liver disease, are characterized by continual inflammation, progressive destruction and regeneration of the hepatic parenchyma, liver progenitor cell proliferation, and fibrosis. The end-stage of every chronic liver disease is cirrhosis, a major risk factor for the development of hepatocellular carcinoma. To study processes regulating disease initiation, establishment, and progression, several animal models are used in laboratories. Here we describe a six-week time course of the choline-deficient and ethionine-supplemented (CDE) mouse model, which involves feeding six-week old male C57BL/6J mice with choline-deficient chow and 0.15% DL-ethionine-supplemented drinking water. Monitoring of animal health and a typical body weight loss curve are explained. The protocol demonstrates the gross examination of a CDE-treated liver and blood collection by cardiac puncture for subsequent serum analyses. Next, the liver perfusion technique and collection of different hepatic lobes for standard evaluations are shown, including liver histology assessments by hematoxylin and eosin or Sirius Red stainings, immunofluorescent detection of hepatic cell populations as well as transcriptome profiling of the liver microenvironment. This mouse model is suitable for studying inflammatory, fibrogenic, and liver progenitor cell dynamics induced through chronic liver disease and can be used to test potential therapeutic agents that may modulate these processes.

    Citation
    Gogoi-Tiwari, J. and Köhn-Gaone, J. and Giles, C. and Schmidt-Arras, D. and Gratte, F. and Elsegood, C. and McCaughan, G. et al. 2017. The murine choline-deficient, ethionine-supplemented (CDE) diet model of chronic liver injury. Journal of Visualized Experiments. 128: Article ID e56138.
    Source Title
    Journal of Visualized Experiments
    URI
    http://hdl.handle.net/20.500.11937/59518
    DOI
    10.3791/56138
    Department
    School of Biomedical Sciences

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