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    Effects of high EPA and high DHA fish oils on changes in signaling associated with protein metabolism induced by hindlimb suspension in rats

    246429_246429.pdf (2.453Mb)
    Access Status
    Open access
    Authors
    Marzuca-Nassr, G.
    Vitzel, K.
    De Sousa, L.
    Murata, G.
    Crisma, A.
    Rodrigues Junior, C.
    Abreu, P.
    Torres, R.
    Mancini-Filho, J.
    Hirabara, S.
    Newsholme, Philip
    Curi, R.
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Marzuca-Nassr, G. and Vitzel, K. and De Sousa, L. and Murata, G. and Crisma, A. and Rodrigues Junior, C. and Abreu, P. et al. 2016. Effects of high EPA and high DHA fish oils on changes in signaling associated with protein metabolism induced by hindlimb suspension in rats. Physiological Reports. 4 (18): pp. e12958.
    Source Title
    Physiological Reports
    DOI
    10.14814/phy2.12958
    School
    School of Biomedical Sciences
    Remarks

    This open access article is distributed under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    URI
    http://hdl.handle.net/20.500.11937/6048
    Collection
    • Curtin Research Publications
    Abstract

    © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.The effects of either eicosapentaenoic (EPA)- or docosahexaenoic (DHA)-rich fish oils on hindlimb suspension (HS)-induced muscle disuse atrophy were compared. Daily oral supplementations (0.3 mL/100 g b.w.) with mineral oil (MO) or high EPA or high DHA fish oils were performed in adult rats. After 2 weeks, the animals were subjected to HS for further 2 weeks. The treatments were maintained alongside HS. At the end of 4 weeks, we evaluated: body weight gain, muscle mass and fat depots, composition of fatty acids, cross-sectional areas (CSA) of the soleus muscle and soleus muscle fibers, activities of cathepsin L and 26S proteasome, and content of carbonylated proteins in the soleus muscle. Signaling pathway activities associated with protein synthesis (Akt, p70S6K, S6, 4EBP1, and GSK3-beta) and protein degradation (atrogin-1/MAFbx, and MuRF1) were evaluated. HS decreased muscle mass, CSA of soleus muscle and soleus muscle fibers, and altered signaling associated with protein synthesis (decreased) and protein degradation (increased). The treatment with either fish oil decreased the ratio of omega-6/omega-3 fatty acids and changed protein synthesis-associated signaling. EPA-rich fish oil attenuated the changes induced by HS on 26S proteasome activity, CSA of soleus muscle fibers, and levels of p-Akt, total p70S6K, p-p70S6K/total p70S6K, p-4EBP1, p-GSK3-beta, p-ERK2, and total ERK 1/2 proteins. DHA-rich fish oil attenuated the changes induced by HS on p-4EBP1 and total ERK1 levels. The effects of EPA-rich fish oil on protein synthesis signaling were more pronounced. Both EPA- and DHA-rich fish oils did not impact skeletal muscle mass loss induced by non-inflammatory HS.

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