Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Effect of human rhinovirus infection on airway epithelium tight junction protein disassembly and transepithelial permeability

    Access Status
    Fulltext not available
    Authors
    Looi, K.
    Troy, N.
    Garratt, L.
    Iosifidis, T.
    Bosco, A.
    Buckley, A.
    Ling, K.
    Martinovich, K.
    Kicic-Starcevich, E.
    Shaw, N.
    Sutanto, E.
    Zosky, G.
    Rigby, P.
    Larcombe, Alexander
    Knight, D.
    Kicic, Anthony
    Stick, S.
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Looi, K. and Troy, N. and Garratt, L. and Iosifidis, T. and Bosco, A. and Buckley, A. and Ling, K. et al. 2016. Effect of human rhinovirus infection on airway epithelium tight junction protein disassembly and transepithelial permeability. Experimental Lung Research. 42 (7): pp. 380-395.
    Source Title
    Experimental Lung Research
    DOI
    10.1080/01902148.2016.1235237
    ISSN
    0190-2148
    URI
    http://hdl.handle.net/20.500.11937/63321
    Collection
    • Curtin Research Publications
    Abstract

    © 2016 Taylor & Francis. Rationale: No studies have assessed the effects of human rhinovirus (HRV) infection on epithelial tight junctions (TJs) and resultant barrier function. Aim of the Study: To correlate viral infection with TJ disassembly, epithelial barrier integrity, and function. Materials and Methods: Human airway epithelial cells were infected with HRV minor serotype 1B (HRV-1B) at various 50% tissue culture infectivity doses (TCID 50 ) over 72 hours. HRV replication was assessed by quantitative-polymerase chain reaction (qPCR) while cell viability and apoptosis were assessed by proliferation and apoptotic assays, respectively. Protein expression of claudin-1, occludin, and zonula occludens protein-1 (ZO-1) was assessed using In-Cell™ Western assays. Transepithelial permeability assays were performed to assess effects on barrier functionality. RT 2 Profiler focused qPCR arrays and pathway analysis evaluating associations between human TJ and antiviral response were performed to identify potential interactions and pathways between genes of interests. Results: HRV-1B infection affected viability that was both time and TCID 50 dependent. Significant increases in apoptosis and viral replication post-infection correlated with viral titer. Viral infection significantly decreased claudin-1 protein expression at the lower TCID 50 , while a significant decrease in all three TJ protein expressions occurred at higher TCID 50 . Decrease in protein expression was concomitant with significant increases in epithelial permeability of fluorescein isothiocynate labeled-dextran 4 and 20 kDa. Analysis of focused qPCR arrays demonstrated a significant decrease in ZO-1 gene expression. Furthermore, network analysis between human TJ and antiviral response genes revealed possible interactions and regulation of TJ genes via interleukin (IL)-15 in response to HRV-1B infection. Conclusion: HRV-1B infection directly alters human airway epithelial TJ expression leading to increased epithelial permeability potentially via an antiviral response of IL-15.

    Related items

    Showing items related by title, author, creator and subject.

    • Effects of human rhinovirus on epithelial barrier integrity and function in children with asthma
      Looi, K.; Buckley, A.; Rigby, P.; Garratt, L.; Iosifidis, T.; Zosky, G.; Larcombe, Alexander; Lannigan, F.; Ling, K.; Martinovich, K.; Kicic-Starcevich, E.; Shaw, N.; Sutanto, E.; Knight, D.; Kicic, A.; Stick, S. (2018)
      Background: Bronchial epithelial tight junctions (TJ) have been extensively assessed in healthy airway epithelium. However, no studies have yet assessed the effect of human rhinovirus (HRV) infection on the expression and ...
    • Azithromycin reduces airway inflammation induced by human rhinovirus in lung allograft recipients
      Ling, K.M.; Hillas, J.; Lavender, M.A.; Wrobel, J.P.; Musk, M.; Stick, S.M.; Kicic, Anthony (2019)
      © 2019 Asian Pacific Society of Respirology Background and objective: Human rhinovirus (RV) is a common upper and lower respiratory pathogen in lung allograft recipients causing respiratory tract exacerbation and contributing ...
    • Therapeutic impact of human serum albumin-thioredoxin fusion protein on influenza virus-induced lung injury mice
      Tanaka, R.; Ishima, Y.; Enoki, Y.; Kimachi, K.; Shirai, T.; Watanabe, H.; Chuang, Victor; Maruyama, T.; Otagiri, M. (2014)
      Reactive oxygen species (ROS) are the primary pathogenic molecules produced in viral lung infections. We previously reported on the use of a recombinant human serum albumin (HSA)-thioredoxin 1 (Trx) fusion protein (HSA-Trx) ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.