Growth, enzymatic glutathione peroxidase activity and biochemical status of juvenile barramundi (Lates calcarifer) fed dietary fermented lupin meal supplemented with organic selenium

Abstract

© 2017 John Wiley & Sons Ltd To investigate the effects of high level of lupin meal (LM) supplemented with organic selenium (OS) on the growth and blood biochemistry of barramundi (Lates calcarifer), four isocaloric and isonitrogenous diets were prepared, containing either non-fermented or fermented LM, and either supplemented with 2 mg OS/kg (LM, LM OS , FLM and FLM OS ), or not. A fishmeal (FM)-based diet formulated for juvenile barramundi was used as a control diet. Fish (initial mean weight of 5.88 g) were triplicated and fed the test diets for 75 days. The findings demonstrated that growth performance of fish fed with the FLM and FLM OS diets were similar to fish fed with the FM diet (p > .05). The antioxidant glutathione peroxidase (GPx) activity, and haemoglobin (Hb) of fish fed with the FLM OS diet were significantly higher than that of FM-fed fish (p < .05). Plasma alanine aminotransferase (ALT) activity was significantly increased in fish fed with non-fermented diets (LM and LM OS ) than in those fed with fermented LM diets (FLM and FLM OS ) (p < .05). However, there were no significant differences in ALT activity among LM OS , FLM, FLM OS and FM diets. There was an interaction between the LM and OS on plasma CK activity; the CK of fish fed with diets supplemented with OS was higher in non-fermented LM diets but lower in fermented LM diets (p < .05). This study suggests that fermented LM have an obvious potential to substantially replace 75% FM protein in the diets of barramundi.