Curtin University Homepage
  • Library
  • Help
    • Admin

    espace - Curtin’s institutional repository

    JavaScript is disabled for your browser. Some features of this site may not work without it.
    View Item 
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item
    • espace Home
    • espace
    • Curtin Research Publications
    • View Item

    Transcriptome analysis of neutrophils after endurance exercise reveals novel signaling mechanisms in the immune response to physiological stress

    Access Status
    Open access via publisher
    Authors
    Neubauer, O.
    Sabapathy, S.
    Lazarus, R.
    Jowett, J.
    Desbrow, B.
    Peake, J.
    Cameron-Smith, D.
    Haseler, Luke
    Wagner, K.
    Bulmer, A.
    Date
    2013
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Neubauer, O. and Sabapathy, S. and Lazarus, R. and Jowett, J. and Desbrow, B. and Peake, J. and Cameron-Smith, D. et al. 2013. Transcriptome analysis of neutrophils after endurance exercise reveals novel signaling mechanisms in the immune response to physiological stress. Journal of Applied Physiology. 114 (12): pp. 1677-1688.
    Source Title
    Journal of Applied Physiology
    DOI
    10.1152/japplphysiol.00143.2013
    ISSN
    8750-7587
    URI
    http://hdl.handle.net/20.500.11937/63514
    Collection
    • Curtin Research Publications
    Abstract

    Neutrophils serve as an intriguing model for the study of innate immune cellular activity induced by physiological stress. We measured changes in the transcriptome of circulating neutrophils following an experimental exercise trial (EXTRI) consisting of 1 h of intense cycling immediately followed by 1 h of intense running. Blood samples were taken at baseline, 3 h, 48 h, and 96 h post-EXTRI from eight healthy, endurance-trained, male subjects. RNA was extracted from isolated neutrophils. Differential gene expression was evaluated using Illumina microarrays and validated with quantitative PCR. Gene set enrichment analysis identified enriched molecular signatures chosen from the Molecular Signatures Database. Blood concentrations of muscle damage indexes, neutrophils, interleukin (IL)-6 and IL-10 were increased (P < 0.05) 3 h post-EXTRI. Upregulated groups of functionally related genes 3 h post-EXTRI included gene sets associated with the recognition of tissue damage, the IL-1 receptor, and Toll-like receptor (TLR) pathways (familywise error rate, P value < 0.05). The core enrichment for these pathways included TLRs, lowaffinity immunoglobulin receptors, S100 calcium binding protein A12, and negative regulators of innate immunity, e.g., IL-1 receptor antagonist, and IL-1 receptor associated kinase-3. Plasma myoglobin changes correlated with neutrophil TLR4 gene expression (r = 0.74; P < 0.05). Neutrophils had returned to their nonactivated state 48 h post-EXTRI, indicating that their initial proinflammatory response was transient and rapidly counterregulated. This study provides novel insight into the signaling mechanisms underlying the neutrophil responses to endurance exercise, suggesting that their transcriptional activity was particularly induced by damage-associated molecule patterns, hypothetically originating from the leakage of muscle components into the circulation. Copyright © 2013 the American Physiological Society.

    Related items

    Showing items related by title, author, creator and subject.

    • Structure and function of the insulin receptor: its role during lactation and foetal development
      Deleo, Domenica (1994)
      Prior to the commencement of this study in 1990, a number of reports had appeared in the literature describing the importance of insulin action during lactation in mammals (see Chapter 1). These studies investigated the ...
    • Neutrophil extracellular traps induce aggregation of washed human platelets independently of extracellular DNA and histones
      Elaskalani, O.; Abdol Razak, N.; Metharom, Pat (2018)
      © 2018 The Author(s). Background: The release of neutrophil extracellular traps (NETs), a mesh of DNA, histones and neutrophil proteases from neutrophils, was first demonstrated as a host defence against pathogens. Recently ...
    • Distinct inflammatory responses differentiate cerebral infarct from transient ischaemic attack
      Armstrong, C.; Bosio, E.; Neil, C.; Brown, S.; Hankey, G.; Fatovich, Daniel (2017)
      We previously reported on a 26-year-old patient who presented early during a large and eventually fatal cerebral infarct. Microarray analysis of blood samples from this patient demonstrated initially up-regulated and ...
    Advanced search

    Browse

    Communities & CollectionsIssue DateAuthorTitleSubjectDocument TypeThis CollectionIssue DateAuthorTitleSubjectDocument Type

    My Account

    Admin

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Follow Curtin

    • 
    • 
    • 
    • 
    • 

    CRICOS Provider Code: 00301JABN: 99 143 842 569TEQSA: PRV12158

    Copyright | Disclaimer | Privacy statement | Accessibility

    Curtin would like to pay respect to the Aboriginal and Torres Strait Islander members of our community by acknowledging the traditional owners of the land on which the Perth campus is located, the Whadjuk people of the Nyungar Nation; and on our Kalgoorlie campus, the Wongutha people of the North-Eastern Goldfields.