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    Diversity of virulence factors associated with West Australian methicillin-sensitive staphylococcus aureus isolates of human origin

    241220_241220.pdf (1.164Mb)
    Access Status
    Open access
    Authors
    Waryah, Charlene Babra
    Gogoi-Tiwari, Jully
    Wells, Kelsi
    Eto, K.
    Masoumi, E.
    Costantino, Paul
    Kotiw, M.
    Mukkur, T.
    Date
    2016
    Type
    Journal Article
    
    Metadata
    Show full item record
    Citation
    Waryah, C.B. and Gogoi-Tiwari, J. and Wells, K. and Eto, K. and Masoumi, E. and Costantino, P. and Kotiw, M. et al. 2016. Diversity of virulence factors associated with West Australian methicillin-sensitive staphylococcus aureus isolates of human origin. BioMed Research International. 2016: Article 8651918.
    Source Title
    BioMed Research International
    DOI
    10.1155/2016/8651918
    ISSN
    2314-6133
    School
    School of Biomedical Sciences
    Remarks

    This open access article is distributed under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    URI
    http://hdl.handle.net/20.500.11937/6475
    Collection
    • Curtin Research Publications
    Abstract

    An extensive array of virulence factors associated with S. aureus has contributed significantly to its success as a major nosocomial pathogen in hospitals and community causing variety of infections in affected patients. Virulence factors include immune evading capsular polysaccharides, poly-N-acetyl glucosamine, and teichoic acid in addition to damaging toxins including hemolytic toxins, enterotoxins, cytotoxins, exfoliative toxin, and microbial surface components recognizing adhesive matrix molecules (MSCRAMM). In this investigation, 31 West Australian S. aureus isolates of human origin and 6 controls were analyzed for relative distribution of virulence-associated genes using PCR and/or an immunoassay kit and MSCRAMM by PCR-based typing. Genes encoding MSCRAMM, namely, Spa, ClfA, ClfB, SdrE, SdrD, IsdA, and IsdB, were detected in >90% of isolates. Gene encoding a-toxin was detected in >90% isolates whereas genes encoding ß-toxin and SEG were detectable in 50-60% of isolates. Genes encoding toxin proteins, namely, SEA, SEB, SEC, SED, SEE, SEH, SEI, SEJ, TSST, PVL, ETA, and ETB, were detectable in >50% of isolates. Use of RAPD-PCR for determining the virulence factor-based genetic relatedness among the isolates revealed five cluster groups confirming genetic diversity among the MSSA isolates, with the greatest majority of the clinical S. aureus (84%) isolates clustering in group IIIa.

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