Impact of mid-season sulphur deficiency on wheat nitrogen metabolism and biosynthesis of grain protein
MetadataShow full item record
© 2018 The Author(s). Wheat (Triticum aestivum) quality is mainly determined by grain storage protein compositions. Sulphur availability is essential for the biosynthesis of the main wheat storage proteins. In this study, the impact of different sulphur fertilizer r egimes on a range of agronomically important traits and associated gene networks was studied. High-performance liquid chromatography was used to analyse the protein compositions of grains grown under four different sulphur treatments. Results revealed that sulphur supplementation had a significant effect on grain yield, harvest index, and storage protein compositions. Consequently, two comparative sulphur fertilizer treatments (0 and 30 kg ha -1 sulphur, with 50 kg ha -1 nitrogen) at seven days post-anthesis were selected for a transcriptomics analysis to screen for differentially expressed genes (DEGs) involved in the regulation of sulphur metabolic pathways. The International Wheat Genome Sequencing Consortium chromosome survey sequence was used as reference. Higher sulphur supply led to one up-regulated DEG and sixty-three down-regulated DEGs. Gene ontology enrichment showed that four down-regulated DEGs were significantly enriched in nitrogen metabolic pathway related annotation, three of which were annotated as glutamine synthetase. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment identified three significantly enriched pathways involved in nitrogen and amino acid metabolism.
Showing items related by title, author, creator and subject.
Associations of NAM-A1 alleles with the onset of senescence and nitrogen use efficiency under Western Australian conditionsAlhabbar, Z.; Islam, S.; Yang, R.; Diepeveen, Dean; Anwar, M.; Balotf, S.; Sultana, N.; Maddern, R.; She, M.; Zhang, J.; Ma, W.; Juhasz, A. (2018)© 2018, Springer Nature B.V. Wheat grain yield and protein content are significantly influenced by the onset of senescence and the duration of the grain filling phase. The onset of senescence also affects Nitrogen use ...
The Fusarium crown rot pathogen Fusarium pseudograminearum triggers a suite of transcriptional and metabolic changes in bread wheat (Triticum aestivum L.)Powell, J.; Carere, J.; Fitzgerald, T.; Stiller, J.; Covarelli, Lorenzo; Xu, Q.; Gubler, F.; Colgrave, M.; Gardiner, D.; Manners, J.; Henry, R.; Kazan, K. (2017)Background and Aims: Fusarium crown rot caused by the fungal pathogen Fusarium pseudograminearum is a disease of wheat and barley, bearing significant economic cost. Efforts to develop effective resistance to this disease ...
The Mak2 MAP kinase signal transduction pathway is required for pathogenicity in Stagonospora nodorumSolomon, P.; Waters, Ormonde; Simmonds, J.; Cooper, R.; Oliver, Richard (2005)A gene encoding a mitogen-activated protein kinase (MAPK) putatively orthologous to Pmk1 from Magnaporthe grisea was cloned and characterised from the wheat glume blotch pathogen Stagonospora nodorum. Protein sequence ...