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dc.contributor.authorAbdo, M.
dc.contributor.authorHisheh, S.
dc.contributor.authorArfuso, Frank
dc.contributor.authorDharmarajan, Arunasalam
dc.date.accessioned2017-01-30T10:54:14Z
dc.date.available2017-01-30T10:54:14Z
dc.date.created2016-09-12T08:36:25Z
dc.date.issued2008
dc.identifier.citationAbdo, M. and Hisheh, S. and Arfuso, F. and Dharmarajan, A. 2008. The expression of tumor necrosis factor-alpha, its receptors and steroidogenic acute regulatory protein during corpus luteum regression. Reproductive Biology and Endocrinology. 6.
dc.identifier.urihttp://hdl.handle.net/20.500.11937/6631
dc.identifier.doi10.1186/1477-7827-6-50
dc.description.abstract

Background: Corpus luteum (CL) regression is known to occur as two parts; functional regression when steroidogenesis declines and structural regression when apoptosis is induced. Previous studies suggest this process occurs by the production of luteolytic factors, such as tumour necrosis factor-alpha (TNF-alpha). Methods: We examined TNF-alpha, TNF-alpha receptors (TNFR1 and 2) and steroidogenic acute regulatory (StAR) protein expression during CL regression in albino Wistar rats. CL from Days 16 and 22 of pregnancy and Day 3 post-partum were examined, in addition CL from Day 16 of pregnancy were cultured in vitro to induce apoptosis. mRNA was quantitated by kinetic RT-PCR and protein expression examined by immunohistochemistry and Western blot analyses. Results: TNF-alpha mRNA increased on Day 3 post-partum. TNFR were immunolocalized to luteal cells, and an increase in TNFR2 mRNA observed on Day 3 post-partum whilst no change was detected in TNFR1 mRNA relative to Day 16. StAR protein decreased on Day 3 post-partum and following trophic withdrawal but no change was observed following exogenous TNF-alpha treatment. StAR mRNA decreased on Day 3 post-partum; however, it increased following trophic withdrawal and TNF-alpha treatment in vitro. Conclusion: These results demonstrate the existence of TNFR1 and TNFR2 in rat CL and suggest the involvement of TNF-alpha in rat CL regression following parturition. Furthermore, decreased StAR expression over the same time points was consistent with the functional regression of the CL. © 2008 Abdo et al; licensee BioMed Central Ltd.

dc.titleThe expression of tumor necrosis factor-alpha, its receptors and steroidogenic acute regulatory protein during corpus luteum regression
dc.typeJournal Article
dcterms.source.volume6
dcterms.source.titleReproductive Biology and Endocrinology
curtin.departmentSchool of Biomedical Sciences
curtin.accessStatusOpen access via publisher


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